Interaction of loratadine with serum albumins studied by fluorescence quenching method |
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Authors: | Zhou Bo Qi Zu-De Xiao Qi Dong Jia-Xin Zhang Ye-Zhong Liu Yi |
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Institution: | Department of Chemical Biology, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China. |
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Abstract: | The interactions between loratadine and bovine serum albumin (BSA) and human serum albumin (HSA) were studied using tryptophan fluorescence quenching method. The fluorescence intensity of the two serum albumins could be quenched 70% at the molar ratio loratadine]:BSA (or HSA)]=10:1. In the linear range (0-50 micromol L(-1)) quenching constants were calculated using Stern-Volmer equation. Temperature in the range 298 K-310 K had a significant effect (p<0.05) on the two serum albumins through ANOVA analysis and t-test. Furthermore the conformation changes in the interactions were studied using FTIR spectroscopy. |
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Keywords: | Loratadine Fluorescence quenching Tryptophan Serum albumin |
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