| HIV-1 gag与gp41基因片段的序列特征与亚型研究 |
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| 引用本文： | 杨成勇,刘翌.HIV-1 gag与gp41基因片段的序列特征与亚型研究[J].病毒学报,2006,22(1):1-5. |
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| 作者姓名： | 杨成勇 刘翌 |
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| 作者单位： | 北京出入境检验检疫局,北京,100013 |
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| 基金项目： | 国家质检总局科研项目(课题编号为2002IK006-02) |
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| 摘 要： | 本文对华北地区出入境39例HIV-1阳性样本(中国21例,非洲17例,东南亚1例)的gag和env两个基因片段进行了序列特征和亚型对比分析。发现了A、A1、A3、B、C、G亚型和重组亚型03_AB、01_AE、AG、02_AG、07_BC、08_BC、CD和06_CPX共14个亚型,其中重组亚型占57.2%(8/14)。表明HIV-1基因变异较快,亚型分布广泛,重组亚型有增多趋势。此外发现26.7%(8/30)的样本,其gag和env基因区亚型表现不一致。提示在研究HIV-1亚型中应综合gag和env两个基因区的序列特征进行亚型分析。
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| 关 键 词： | HIV-1 逆转录PCR(RT-PCR) 系统树 亚型 |
| 文章编号： | 1000-8721（2006）01-0001-05 |
| 收稿时间： | 2005-10-26 |
| 修稿时间： | 2005-10-31 |
Study on Subtypes of HIV-1 gag and gp41 Gene Segments |
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| YANG Cheng-yong,LIU Yi.Study on Subtypes of HIV-1 gag and gp41 Gene Segments[J].Chinese Journal of Virology,2006,22(1):1-5. |
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| Authors: | YANG Cheng-yong LIU Yi |
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| Institution: | Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing 100013, China |
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| Abstract: | Subtype diversity of gag and env gene segments of HIV-1 isolates from Exit-Entry people of North China,including 21 Chinese,17 African and 1 Southeast Asian were studied.Thirty nine of the newly sequenced Chinese isolates could be assigned to subtypes A,A1,A3,B,C,G,03.AB,01.AE,AG,02.AG,07.BC,08.BC,CD and 06.CPX.Of the total 14 subtypes,57.2%(8/14)subtypes showed recombinant gene structure.Eight of thirty samples(26.7%) had discordant subtype designations between gag and env genes.It suggested that HIV-1 genes varied fast and recombinant subtypes increased greatly.Subtype analysis of HIV-1 should better be taken both the gag and env gene segments in consideration. |
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| Keywords: | HIV-1 RT-PCR phylogenetic tree subtype |
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