| 乙型肝炎病毒聚合酶基因B区和C区序列的异质性 |
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| 引用本文： | 王战会,孙剑,侯金林,陈金军,肖蕾,骆抗先.乙型肝炎病毒聚合酶基因B区和C区序列的异质性[J].病毒学报,2000,16(1):61-64. |
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| 作者姓名： | 王战会 孙剑 侯金林 陈金军 肖蕾 骆抗先 |
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| 作者单位： | 第一军医大学南方医院感染内科,广东,广州,510515 |
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| 基金项目： | 国家自然科学基金，广东省自然科学基金 |
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| 摘 要： | 乙型肝炎病毒(HBV)聚合酶基因编码约816个氨基酸的多蛋白,其氨基端为末端蛋白,羧基端为RNA酶H,后者上游紧邻反转录酶/DNA聚合酶.通过计算机模拟分析将HBV聚合酶基因分为假想的A到E共5个区段.
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| 关 键 词： | 乙型肝炎病毒 聚合酶基因 异质性 |
Heterogeneity in B and C Domains of Polymerase Gene of Hepatitis B Virus |
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| WANG Zhan-hui,SUN Jian,HOU Jin-lin,CHEN Jin-jun,XIAO Lei,LOU Kang-xian.Heterogeneity in B and C Domains of Polymerase Gene of Hepatitis B Virus[J].Chinese Journal of Virology,2000,16(1):61-64. |
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| Authors: | WANG Zhan-hui SUN Jian HOU Jin-lin CHEN Jin-jun XIAO Lei LOU Kang-xian |
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| Abstract: | By the method of PCR product sequencing and cloning sequencing,we sequenced the B and C domains of HBV polymerase gene of the serum samples from 27 hepatitis B surface antigen positive patients and 7 hepatitis B surface antigen negative patients. At the same time, we compared the B and C domains of polymerase gene with that of 8 reported HBV complete sequences. We found that some amino acids are highly conserved. However, there are naturally mutated substitutions in other amino acids. The results showed that the polymerase gene are heterogeneous. It can act as a reference for the research of nucleotide related mutations of polymerase gene. |
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| Keywords: | hepatitis B virus polymerase gene heterogeneity |
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