| 大麦条纹花叶病毒新疆分离物是三组分RNA病毒 |
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| 引用本文： | 彭学贤,蔡发兴,孙薇,莽克强.大麦条纹花叶病毒新疆分离物是三组分RNA病毒[J].病毒学报,1986(3). |
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| 作者姓名： | 彭学贤 蔡发兴 孙薇 莽克强 |
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| 作者单位： | 中国科学院微生物研究所 北京
(彭学贤，蔡发兴，孙薇)，中国科学院微生物研究所 北京(莽克强) |
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| 摘 要： | 大麦条纹花叶病毒新疆分离物(BSMV-XJ)的RNA在乙二醛,二甲亚砜中进行变性电泳,电泳图谱可重复的显示三个组分。将电泳分离的BSMV-RNA各组分在高盐浓度下吸附转移到硝酸纤维素薄膜上,经固定后,与~(32)P标记的病毒总RNA的cDNA行分子杂交。在X光放射自显影图谱上,进一步证实BSMV-XJ为三组分RNA病毒。本文同时就BSMV-RNA不经变性电泳也可以部分吸附转移到硝酸纤维素薄膜上的现象讨论了可能的分子基础。
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| 关 键 词： | 大麦条纹花叶病毒 多组分RNA病毒 Northern吸印法 变性和非变性RNA凝胶电泳 |
BARLEY STRIPE MOSAIC VIRUS XJ ISOLATE IS A TRIPARTITE VIRUS |
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| Peng Xuexian Cai Faxing Sun Wei Mang Keqiang.BARLEY STRIPE MOSAIC VIRUS XJ ISOLATE IS A TRIPARTITE VIRUS[J].Chinese Journal of Virology,1986(3). |
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| Authors: | Peng Xuexian Cai Faxing Sun Wei Mang Keqiang |
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| Abstract: | A reproducible agarose electrophoretic patten with BSMV-RNAs denatured in glyoxal and DMSO shows that the Barley Stripe Mosaic Virus XJ isolate is a tripartite virus. The three denatured RNA components separated by elect-rophoresis were transferred onto nitrocellulose papers and hybridized with the 32P labelled cDNA probes synthesized from BSMV's total RNA. Autoradiogra-phy further shows that BSNV-XJ isolate,like Norwich Strain or Norta Dakota 18?is a three RNA-components containing virus.We also discussed the possible molecular basis for the different transfer efficiency onto the nitrocellulose paper of the different RNA components without denaturation. |
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| Keywords: | BSMV Multicomponent RNA virus Northern blot Agarose gel electrophoresis of RNA under denatured or noadenatured condition |
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