新成人腹泻轮状病毒J19株VP2、VP3的编码基因序列分析

利用一种改良的非依赖核酸序列的单引物扩增方法,从新成人腹泻轮状病毒J19株的核酸中扩增基因,克隆至pMD18-T中并进行测序和基因序列分析。J19株的VP2、VP3的编码基因为基因2、4,分别长2 969bp、2 204bp,它们分别编码973个氨基酸和719个氨基酸。J19株的VP2蛋白序列对B组人轮状病毒IDIR株的一致性为47.2%;J19株的VP3蛋白序列对C组人轮状病毒Cowden株一致性为25.1%。对J19株VP2的遗传进化分析表明,J19株在进化树上的位置靠近外群蛋白以及A、B和C组轮状病毒分枝的根部,并且它比较偏向于B组轮状病毒的分枝。这与VP6的遗传进化分析结果相一致。根据上述结果推测J19株可能是一个与B组轮状病毒的起源和进化密切相关的毒株之一;同时,这表明VP2在研究轮状病毒的遗传进化上具有重要价值。关于新成人腹泻轮状病毒J19株VP2、VP3的编码基因的序列分析,这是首次报道。

Sequence Analysis of the VP2 and VP3 Genes of a Novel Adult Diarrhea Rotavirus Strain J19

Genes of a novel adult diarrhea rotavirus(NADRV) strain J19 were amplified by an improved single primer sequence-independent amplification method,cloned into pMD18-T vector,sequenced and analyzed.Genomic segments 2,4 encoding VP2,VP3 of NADRV-J19 were 2969bp,2204bp in length,and the deduced proteins were 973aa and 719aa,respectively.Identity of VP2 of J19 to human group B rotavirus strain IDIR was 47.2 % and that of VP3 of J19 to human group C rotavirus strain Cowden was 25.1%.Phylogenetic analysis of VP2 showed that NADRV-J19 positioned closely to outgroup and the ancestral nodes of group A,B and C lineages, although with a preferred association with group B lineage,which corresponded to the phylogenetic analysis of VP6.The above results allowed us to deduce a conclusion that J19 would be a strain closely related to the evolution and origin of group B rotaviruses and they also indicated that VP2 would be valuable for studying the evolution and origin of rotaviruses.This study is the first report on sequence analysis of genes of NADRV-J19 encoding VP2 and VP3.