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以杆状病毒为载体在昆虫细胞中表达鸡马立克病病毒糖蛋白B抗原基因
引用本文:李毅,崔治中.以杆状病毒为载体在昆虫细胞中表达鸡马立克病病毒糖蛋白B抗原基因[J].病毒学报,1994,10(1):24-32.
作者姓名:李毅  崔治中
作者单位:江苏农学院
摘    要:

关 键 词:马立克病病毒  糖蛋白B抗原  载体

EXPRESSION OF MAREKS DISEASE VIRUS gS GENE IN INSECT CELLS USING BACULOVIRUS AS A VECTOR
Li Yi Cui Zhizhong Sui Dexin Lee L.F..EXPRESSION OF MAREKS DISEASE VIRUS gS GENE IN INSECT CELLS USING BACULOVIRUS AS A VECTOR[J].Chinese Journal of Virology,1994,10(1):24-32.
Authors:Li Yi Cui Zhizhong Sui Dexin Lee LF
Abstract:y using baculovirus AcNPV as a vector,the Marek's disease virus(MDV)gB gene,which was closely related to virus-neutralization, was expressed in the insect cell line Sf9 cells.In the immunoprecipitation of 35S-methionine labeled recombinnt gB-AcNPV infected Sf9cell lysates,both anti-MDV gB monoclonal antibody and anti-MDV chicken serarecognized 3 MDV-specific protein bands of 98kD, 55kD ,and 49kD. which were very similarto the native MDV gB of 100kD, 60kD and 49kD in the sizes and patterns. Treatment ofgB-AcNPV infected Sf9 cells with tunicamycin during the labeling or with N-glycanase andEndo-H after labeling for immunoprecipitation indicated that the recombinant gB(r-gB)wasglycosylated in insect cells as the native gB in the MDV-infected chink embryo fibroblast(CEF)ell system. The r-gB and native gB were very similar although there was some differences between them in the degree and sites for glycosylation. The chicken sera against the r-gB gave positive reaction to MDV-CEF in fluorescence antibody test but failed to show virus-neutralizing activity as anti-MDV chicken sera did, the possible reasons were discussed.
Keywords:Marek's disease virus  Glycoprotein B gene  Baculovirus vector  ImmunoprecipitationAvian Disease and Oncology Laboratory  USDA  
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