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应用ELISA方法检测四种呼吸道病毒感染的IgG抗体
引用本文:郑浩强,张群.应用ELISA方法检测四种呼吸道病毒感染的IgG抗体[J].病毒学报,1990,6(1):68-73.
作者姓名:郑浩强  张群
作者单位:中国预防医学科学院病毒学研究所,北京市崇文区儿童医院,中国预防医学科学院病毒学研究所 北京,北京
摘    要:

关 键 词:呼吸道病毒  合胞病毒  副流感病毒

DETECTION OF SERUM IgG ANTIBODIES AGAINST FOUR RESPIRATORY VIRUSES BY AN INDIRECT ELISA
Zheng Haoqiang Zhang Qun Zhang Libi.DETECTION OF SERUM IgG ANTIBODIES AGAINST FOUR RESPIRATORY VIRUSES BY AN INDIRECT ELISA[J].Chinese Journal of Virology,1990,6(1):68-73.
Authors:Zheng Haoqiang Zhang Qun Zhang Libi
Abstract:An indirect ELISA was set up for the detection of specific seru m IgG antibodies to respiratory syncytial virus(RSV), parainfluenza virus(PFV), adenovirus ( AdV ) and measles virus. The antigens used in the ELISA were treated with DOC detergent. Four viral IgG antibodies can be detected at the same time in one serum specimen under testing.The kinetics of IgG antibodies was observed in children with the 4 viral lower respiratory tract infections.Of the 50 pairs of acute and convalescent sera from children with acute lower respiratory infections, 16 ( 32% ) were found positive for IgG to RSV, 10 ( 20% ) to PFV,10 ( 20% ) to AdV and 8 (l6%)to measles virus. In addition, combined infections by two or more viruses were also noted.
Keywords:Respiratory syncytial virus Parainfluenza virus Adenovirus Measles virus ELISA
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