| 细胞周期蛋白依赖性蛋白激酶2在单纯疱疹病毒复制中的作用 |
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| 引用本文： | 关泽红,旭日干.细胞周期蛋白依赖性蛋白激酶2在单纯疱疹病毒复制中的作用[J].病毒学报,2008,24(2):96-100. |
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| 作者姓名： | 关泽红 旭日干 |
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| 作者单位： | 内蒙古大学,哺乳动物生殖生物学与生物技术教育部重点实验室,呼和浩特,010021;内蒙古医学院,生物化学与分子生物学教研室,呼和浩特,010059;内蒙古大学,哺乳动物生殖生物学与生物技术教育部重点实验室,呼和浩特,010021 |
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| 摘 要： | 细胞周期蛋白依赖性蛋白激酶(CDK)与单纯疱疹病毒(HSV)等多种重要人类疾病病毒的复制密切相关.但具体哪种CDK是病毒复制所必需的还不清楚.本文用不同剂量的HSV-1-KOS株(以下简称HSV)感染CDK2功能缺陷型宿主细胞,结果发现HSV在CDK2功能缺陷型宿主细胞中的复制具有感染剂量依赖性;一步生长曲线分析结果表明其在CDK2功能缺陷型宿主细胞中的复制较在正常细胞延迟3h;感染6h时CDK2活性被诱导,9h时活性最大;CDK2活性增加后HSV-1即进入快速的裂解性复制.提示CDK2可能在HSV复制的启动中起着某种重要作用.
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| 关 键 词： | HSV CDK2 病毒-宿主细胞相互作用 |
| 文章编号： | 1000-8721(2008)02-0096-05 |
| 修稿时间： | 2007年9月6日 |
The Role of Cyclin-dependent Protein Kinase 2 in the Replication of Herpes Simplex Virus |
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| GUAN Ze-hong,Shorgen.The Role of Cyclin-dependent Protein Kinase 2 in the Replication of Herpes Simplex Virus[J].Chinese Journal of Virology,2008,24(2):96-100. |
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| Authors: | GUAN Ze-hong Shorgen |
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| Institution: | The Key Laboratory of Mammal Reproductive Biology and Biotechnology, Ministry of Education /Inner Mongolia University, Huhhot 010021, China. guanzehong@yahoo.com.cn |
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| Abstract: | Cyclin-dependent protein kinase(CDK)plays an important role in the replication of herpes simplex virus (HSV) and other important human disease viruses. But which kinds of CDK are required in the replication of HSV is still not clear. In this study, we infected dominant negative CDK2 cell line with different multiplicity of infection (MOI) of HSV-1-KOS strain(abbreviated as HSV below), and the results showed that the yield of HSV depended on the MOI; the replication of HSV delayed about 3 h as compared with that of the control in the one-step growth curve replication experiments; the CDK2 activity was induced 6 h post HSV infection and reached the highest 9 h post infection; the HSV went into rapid productive replication after the CDK2 was induced. We propose herein that the CDK2 is required in the initiation of replication of HSV. |
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| Keywords: | HSV CDK2 |
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