| 山东省甲型H1N1流感病毒病原学及基因特性研究 |
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| 引用本文： | 刘倜,林艺,张圣洋,王爽,尹玉岩,李忠,王显军,徐爱强,毕振强.山东省甲型H1N1流感病毒病原学及基因特性研究[J].病毒学报,2011,27(3):224-230. |
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| 作者姓名： | 刘倜 林艺 张圣洋 王爽 尹玉岩 李忠 王显军 徐爱强 毕振强 |
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| 作者单位： | 山东省疾病预防控制中心山东省传染病预防控制重点实验室,山东大学预防医学研究院,济南,250014;山东大学公共卫生学院,济南,250012 |
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| 基金项目： | 山东省科技厅计划项目(2009GG10002054) |
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| 摘 要： | 在2009~2010年监测年度开展甲型H1N1流感病毒学监测并进行病原学分离鉴定,以及对血凝素基因(HA)和神经氨酸酶基因(NA)特性分析,研究其基因变异情况。采集了17 126份发热患者的鼻、咽拭子标本,采用逆转录实时荧光定量RT-PCR(Real-Time RT-PCR)进行核酸检测,其中甲型H1N1流感病毒核酸检测阳性4004份,总阳性率为23.38%。对阳性标本开展病毒分离,并对分离的甲型H1N1流感病毒的HA、NA基因序列进行测序。利用DNAStar软件对序列进行同源性分析发现与WHO推荐的疫苗株相比,山东省甲型H1N1流感流行株HA、NA基因同源性分别为96.9%~99.3%和99.1%~99.6%;利用Mega 4.0软件进行基因进化分析和氨基酸进化分析发现,与WHO推荐的疫苗株相比,山东省甲型H1N1流感流行株有21个血凝素基因的氨基酸发生替换,其中11个氨基酸位点位于抗原决定簇区,一个糖基化位点发生改变;有16个神经氨酸酶基因的氨基酸发生了替换,一个糖基化位点发生改变;未发生神经氨酸酶蛋白275位H→Y的替换。结果显示山东省甲型H1N1流感暴发流行株HA基因和NA基因均具有高度同源性,HA蛋白和NA蛋白均存在不同程度的氨基酸替换,部分流行株抗原决定簇和糖基化位点发生改变,所有病毒均对达菲类药物敏感。
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| 关 键 词： | 流感病毒 HA基因 NA基因 |
Etiological and molecular characteristics of pandemic influenza A (H1N1) virus during 2009-2010 in Shandong Province |
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| Liu Ti,Lin Yi,Zhang Sheng-Yang,Wang Shuang,Yin Yu-Yan,Li Zhong,Wang Xian-Jun,Xu Ai-Qiang,Bi Zhen-Qiang.Etiological and molecular characteristics of pandemic influenza A (H1N1) virus during 2009-2010 in Shandong Province[J].Chinese Journal of Virology,2011,27(3):224-230. |
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| Authors: | Liu Ti Lin Yi Zhang Sheng-Yang Wang Shuang Yin Yu-Yan Li Zhong Wang Xian-Jun Xu Ai-Qiang Bi Zhen-Qiang |
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| Institution: | LIU TI1,LIN Yi1,ZHANG Sheng-yang1,WANG Shuang1,YIN Yu-yan2,LI ZHONG1,WANG Xian-jun1 XU Ai-qiang1,BI Zhen-qiang1(1.Shandong Center for Disease Control and Prevention,Shandong Provincial Key Laboratory of Disease Control and Prevention,Shandong University Institute for Prevention Medicine,Jinan 250014,China,2.School of Public Health,Shandong University,Jinan 250012,China) |
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| Abstract: | To isolate and identify the influenza virus in Shandong Province in 2009-2010 and analyze the genetic characteristics of hemagglutinin and neuraminidase gene,further study the variation of gene.A total of 17 126 nasopharyngeal swabs from fever patients were collected and detected by real time quantitative RT-PCR method.The results showed 4004 samples were pandemic influenza A(H1N1) virus positive,with an overall positive rate as 23.38%.The positive samples were incubated and cultured in MDCK cells.The HA an... |
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| Keywords: | influenza virus HA gene NA gene |
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