传染性法氏囊病病毒VP3抗原表位的鉴定

利用肽扫描技术对4株IBDV VP3的单克隆抗体(HRB-3F、HRB-7B、HRB-7C和HRB-10E)的抗原表位进行了研究.通过Western blot和ELISA鉴定,将HRB-3F和HRB-7B的抗原表位定位于VP3 109～119 aa(位于IB-DV聚合蛋白的864～874 aa),HRB-7C和HRB-10E的抗原表位定位于VP3 177～190 aa(位于IBDV聚合蛋白的932～945 aa).进一步检测其反应原性及免疫原性,结果表明,这两个表位均能与抗IBDV阳性血清反应.将这两个表位短肽免疫BALB/c小鼠,其血清可以和IBDV反应,具有较好的免疫原性.与D6948、HK46和UK661等多株IBDV相应区域的同源性进行了比较,结果显示,这两个表位在多种毒株中同源性为100%.通过IBDV VP3抗原表位的研究,筛出两个新的保守线性表位并进行精确定位,对进一步分析IBDV结构与功能以及建立以表位为基础的抗原抗体诊断方法具有重要的意义.

Identification of VP3 Antigenic Epitopes of Infectious Bursal Disease Virus

Infectious bursal disease virus(IBD) causes infectious bursal disease(IBD),which infects bursal of chicken and can evoke immune suppression.This study identified the antigenic epitopes of four McAbs to IBDV VP3(HRB-3F,HRB-7B,HRB-7C and HRB-10E)with pepscan.A set of 17 partially overlapping or consecutive peptides(P1-P17) spanning VP3 were expressed for epitope screening by pepscan.Finally,two antigenic epitopes,109-119aa and 177-190aa of IBDV VP3,were identified by Western blot and ELISA.The peptides on epitopes could react with IBDV,and they had better immunnogenicity.The sequences of epitopes were compared with that of several other IBDV strains in the same region,and was found they were totally homologous.This study showed the two epitopes were novel conserved linear B cell epitopes on the VP3 of IBDV.This study provides basis for the development of immunity-based prophylactic,therapeutic and diagnostic measures for control of IBD and further for structural and functional analysis of IBDV.