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黄瓜花叶病毒衣壳蛋白基因转化辣椒研究
引用本文:李华平,胡晋生,王敏,范怀忠.黄瓜花叶病毒衣壳蛋白基因转化辣椒研究[J].病毒学报,2000,16(3):276-278.
作者姓名:李华平  胡晋生  王敏  范怀忠
作者单位:1. 华南农业大学资环学院,广东,广州,510642
2. 夏威夷大学植病系,美国,96822
基金项目:教育部"优秀青年教师资助计划";200011;
摘    要:The plant expression vector of the coat protein(CP) gene of cucumber mosaic virus (CMV) BS strain was used to transform three kinds of pepper (Capsicum annuum) tissues (cotyledon, stem and root) by agrobacterium-mediated co-cultivation. 53%-68.4% of the total tissues (639) can be induced to be calli, but only cotyledon calli can be further regenerated to form shoots (regenerated efficiency 39.7%). 70%(42/60) of the putative transformed plants were confirmed to have CP gene in their genomes by Southern blot. The mRNAs and the CP were respectively found in 80% of transgenic plants by Northern blot and DAS-ELISA. 24 of the transgenic plants expressing CP gene of BS strain showed three kinds of resistant level (severe symptom, delay of symptomatic development, no symptom) to infection of CMV-BS and of CMV-P. However, there was distinctly higher resistance to inoculation of CMV-BS than that with CMV-P in these transgenic plants.

关 键 词:黄瓜花叶病毒  辣椒  衣壳蛋白  表达与抗性

Studies on Transgenic Pepper Plants Transferred with the Coat Protein Gene of Cucumber Mosaic Virus
LI Hua-ping,FAN Huai-zhong,John S Hu,WANG Ming.Studies on Transgenic Pepper Plants Transferred with the Coat Protein Gene of Cucumber Mosaic Virus[J].Chinese Journal of Virology,2000,16(3):276-278.
Authors:LI Hua-ping  FAN Huai-zhong  John S Hu  WANG Ming
Abstract:
Keywords:Cucumber mosaic virus  pepper  coat protein gene(CP)  expression and resistance
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