| 禽流感病毒A/Chicken/Guangdong/SS/94(H9N2)HA基因的克隆及序列分析 |
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| 引用本文： | 郭霄峰,廖明,辛朝安,程小雯.禽流感病毒A/Chicken/Guangdong/SS/94(H9N2)HA基因的克隆及序列分析[J].病毒学报,2002,18(1):80-81. |
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| 作者姓名： | 郭霄峰 廖明 辛朝安 程小雯 |
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| 作者单位： | 1. 华南农业大学,动物医学系,广东,广州,510642;2. 深圳市卫生防疫站,广东,深圳,518020 |
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| 基金项目： | 国家重点基础研究发展计划(973计划);G199901/900; |
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| 摘 要： | 禽流感是由A型流感病毒引起的禽的一种疾病综合症.1878年首次在意大利爆发流行,当时称该病为"鸡瘟".之后,许多国家和地区都有该病的报道,包括美国、英国、澳大利亚、爱尔兰、比利时、荷兰、法国、俄罗斯、加拿大、以色列、匈牙利、日本、中国(包括香港)等1-3].
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| 关 键 词： | H9N2亚型 禽流感病毒 HA基因 序列分析 |
| 文章编号： | 1000-8721(2002)01-0080-03 |
Cloning and Sequencing of HA Gene of Influenza Virus A/Chicken/Guangdong/SS/94(H9N2) |
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| GUO Xiao feng ,LIAO Ming ,XIN Chao an ,CHENG Xiao wen.Cloning and Sequencing of HA Gene of Influenza Virus A/Chicken/Guangdong/SS/94(H9N2)[J].Chinese Journal of Virology,2002,18(1):80-81. |
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| Authors: | GUO Xiao feng LIAO Ming XIN Chao an CHENG Xiao wen |
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| Institution: | GUO Xiao feng 1,LIAO Ming 1,XIN Chao an 1,CHENG Xiao wen 2 |
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| Abstract: | RT-PCR was employed to amplify the cDNA of HA gene of influenza A/Chicken/Guangdong/SS/94(H 9N 2)with a pair of degenerate primers and the cDNA were cloned into the T-T windows of plasmid pMD18-T.The inserts were sequenced,at first time,and the results revealed that the HA gene had a long complete open reading frame and composed of 1,683 nucleotides,coding for 560 amino acids.The amino acid sequences of the HA connecting peptide revealed that A/Chicken/Guangdong/SS/94(H 9N 2)had X-X-X-R(X,not basic amino acid)at the proteolytic cleavage site.The molecular basis of the HA gene was compatible with not highly pathogenicity.Comparison of the degree of homology of HA gene showed 82%-98% nucleotide sequence and amino acid sequence homology among the isolate and the other H 9N 2 subtype AIV in GenBank. Thus, the HA gene of influenza A/Chicken/Guangdong/SS/94 belonged to H 9 subtype. |
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| Keywords: | H 9N 2 subtype avian influenza virus hemagglutinin gene sequencing |
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