HIV-1抗体蛋白印迹确认与核酸检测复核对比研究

应用病毒核酸载量法NASBA和HIV-1 RNA的巢式逆转录PCR(nested RT-PCR)法与HIV抗体蛋白印迹(WB)方法,对经过初筛的44例HIV-1抗体阳性标本进行了对照检测研究。发现了2例(gp160、p24)和1例(gp160g、p120p、66、p24)的特殊阳性样本,经NASBA法和该RT-PCR法核酸检测为阴性;WB确认的4例gp160阳性带、1例p24、p17阳性带和13例p24阳性带,经NASBA法和该RT-PCR法核酸检测也为阴性;而WB确认的其余全部带型的抗体阳性标本经过NASBA法和该RT-PCR法检测均为阳性。该研究表明对只有gp160p、24和gp160、gp120p、66、p24的特殊阳性标本和以p24为主的抗体不确定标本需要用RT-PCR或NASBA方法进行核酸检测,以进一步确认。

Comparative Study of Western Blot Test and Nucleic Acid Detection for HIV-1 Infection

The NASBA,RT-PCR and Western blot(WB) methods were compared for confirmation of 44 HIV-1 samples which had been tested positive firstly by ELISA screening.Among the three specific antibody positive samples by WB test,two samples(gp160,p24) and one sample(gp160,gp120,p66,p24) were tested negative by NASBA and RT-PCR methods.In the eighteen indeterminate samples of WB test,four samples(gp160),one sample(p24,p17) and thirteen samples(p24) were all tested negative by NASBA and RT-PCR methods too.But for the twenty-three real antibody positive samples by WB test(including WB 7 bands),all were shown positive by the NASBA and RT-PCR methods.These results showed that NASBA and RT-PCR methods should be adopted for further identification of the specific Ab positive and Ab indeterminate HIV-1 samples after WB test.