| 庚型肝炎病毒NS3蛋白在昆虫细胞中的表达 |
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| 引用本文： | 朱分禄,任浩,朱诗应,宋燕斌,戚中田.庚型肝炎病毒NS3蛋白在昆虫细胞中的表达[J].病毒学报,2000,16(3):273-275. |
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| 作者姓名： | 朱分禄 任浩 朱诗应 宋燕斌 戚中田 |
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| 作者单位： | 第二军医大学微生物学教研室,上海,200433 |
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| 基金项目： | 国家自然科学基金;39825116,39770393; |
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| 摘 要： | 庚型肝炎病毒(HGV)/GB病毒C(GBV-C)疑似引起人类庚型肝炎1～3].HGV和GBV-C为同一病毒的两个不同分离株,本文将其称为GBV-C/HGV.GBV-C/HGV属黄病毒科,为单股正链RNA病毒,全长约9.4kb.基因组中仅含有一个单一开放阅读框,编码E1、E2结构蛋白和NS2、NS3、NS4及NS5非结构蛋白.GBV-C/HGV的NS3蛋白具备丝氨酸蛋白酶活性和解旋酶活性3],在NS3蛋白中还存在线性抗原表位4],因此,NS3蛋白是GBV-C/HGV的重要功能蛋白.
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| 关 键 词： | GBV-C/HGV NS3蛋白 Sf9昆虫细胞 表达 |
High-level Expression of GBV-C/HGV NS3 Protein in Sf9 Insect Cells Using Bac-to-Bac Vectors |
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| ZHU Fen-lu,REN Hao,ZHU Shi-ying,SONG Yan-bin,QI Zhong-tian.High-level Expression of GBV-C/HGV NS3 Protein in Sf9 Insect Cells Using Bac-to-Bac Vectors[J].Chinese Journal of Virology,2000,16(3):273-275. |
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| Authors: | ZHU Fen-lu REN Hao ZHU Shi-ying SONG Yan-bin QI Zhong-tian |
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| Abstract: | The recombinant GBV-C/HGV NS3 protein was expressed in Sf9 insect cells and analyzed by SDS-PAGE. A protein band about Mr 43810 was demonstrated on polyacrylamide gel electrophoresis and this protein amounted to more than 30% of the total cell proteins. Recombinant NS3 protein was purified by Ni-NTA column. Western blot showed that this recombinant protein could react with GBV-C/HGV RNA positive mixed sera. The recombinant GBV-C/HGV NS3 protein could be used to detect GBV-C/HGV infection and will supply material for study of structure and function of this protein. |
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| Keywords: | GBV-C/HGV NS3 protein Sf9 insect cell expression |
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