| 番茄Tm-22基因在烟草中的表达及其对番茄花叶病毒(ToMV)的特异抗性 |
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| 引用本文： | 姜国勇,杨仁崔.番茄Tm-22基因在烟草中的表达及其对番茄花叶病毒(ToMV)的特异抗性[J].病毒学报,2003,19(4):365-370. |
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| 作者姓名： | 姜国勇 杨仁崔 |
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| 作者单位： | 1. 福建农林大学,福建,福州,350002;莱阳农学院,山东,莱阳,265200
2. 福建农林大学,福建,福州,350002 |
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| 基金项目： | 山东省30工程资助项目;; |
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| 摘 要： | Tm-2^2基因在烟草上的转化和表达表明，Tm-2^2基因在同科不同属植物体上的功能没有改变。在两种类型的纯合转化体上，Tm-2^2基因编码蛋白能够抗Tobamovirus属5种不同的毒株，并能被ToMV-2a毒株感染而失去抗病性。这个结果表明：移动蛋白上的氨基酸变异能够影响R蛋白对ToMV的应答反应。Tm-2^2基因转化体在不同启动子的调控下，对ToMV-2a毒株感染所表现的症状不同，说明启动子在Tm-2^2基因的抗病反应中具有非常重要的作用。
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| 关 键 词： | Tm-2^2基因 番茄 转基因烟草 番茄花叶病毒 启动子 |
| 文章编号： | 1000-8721(2003)04-0365-06 |
Transgenic Tobacco Plants Expressing Tomato Tm-22 Display Resistance Specificity to Tobamoviruses |
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| JIANG Guo-yong.Transgenic Tobacco Plants Expressing Tomato Tm-22 Display Resistance Specificity to Tobamoviruses[J].Chinese Journal of Virology,2003,19(4):365-370. |
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| Authors: | JIANG Guo-yong |
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| Institution: | JIANG Guo-yong~ |
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| Abstract: | The fact that the Tm-2~2 g ene expressed in the transgenic to bacco suggested that the Tm-2~2 res istance property functioned comple tely without any change in diffe rent genera of the Solanoideae fam ily.Tm-2~2-transformed tobacco plan ts were resistant to 5 strains of T obamoviruses and the resistance wa s overcome by the virus breaker To MV-GM65.This result indicated that amino acid variation in the movement protein of ToMV could affect the re sponse activity of R protein to ToMV infection.The infection symptoms d iffering in two types of transform ant suggested that the promoter co ntrolling the expression of Tm-2~2 gene played a significant role in the resistant reaction. |
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| Keywords: | Tm-2~2 gene transgenic to bacco tomato mosaic virus promoter |
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