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肾综合征出血热病毒Gou3株M片段的分子特性及序列的比较分析
引用本文:姚智慧,朱智勇.肾综合征出血热病毒Gou3株M片段的分子特性及序列的比较分析[J].病毒学报,1999,15(3):199-204.
作者姓名:姚智慧  朱智勇
作者单位:中国药品生物制品检定所
摘    要:为测定我国肾综合征出血热病毒Gou3株的M片段全基因序列,了解该株分子基础,并分析其瑟其他病毒株的差异,将提取的感染了病毒的Vero-E6细胞总RNA进行逆转录PCR扩增,产物直接或纯化后克隆T载体并测定序列,结果测得Gou3株M片段的全基因序列共3651个核苷 ,最大读码框架从47到3448,共编码1134个氨基酸。

关 键 词:肾综合征出血热  病毒  Gou3株  M片段  序列

MOLECULAR CHARACTERIZATION AND SEQUENCE COMPARISON OF THE M SEGMENT OF HANTAVIRUS Gou3 STRAIN
Yao Zhihui,Dong Guanmu,Yu Yongxin,Liu wenxue,Zhu Zhiyong.MOLECULAR CHARACTERIZATION AND SEQUENCE COMPARISON OF THE M SEGMENT OF HANTAVIRUS Gou3 STRAIN[J].Chinese Journal of Virology,1999,15(3):199-204.
Authors:Yao Zhihui  Dong Guanmu  Yu Yongxin  Liu wenxue  Zhu Zhiyong
Institution:Yao Zhihui Dong Guanmu Yu Yongxin Liu wenxue Zhu Zhiyong *
Abstract:In order to understand the molecular characters of Hantavirus Gou3 strain, its complete M genome was sequenced and compared with 10 strains of HTN and 10 strains of SEO viruses. We prepared the total RNA from Gou3 virus infected cells and the raw or purified RT-PCR product was cloned and sequenced. The Gou3 strain complete M genome is 3 651 nucleotides in length with a single open reading frame extending from nt47 to 3 448 encoding 1 134 amino acids. It shows only 70.5%-71.5% homologous with HTN viruses at the nucleotide level and 76.5%-77.3% at the amino acid level, 83.9%-84.5% homologous with SEO viruses at the nucleotide level and 93.2%-97.3% at the amino acid level. The Gou3 virus has 29 unique amino acids as compared with other 9 SEO viruses. The results of nucleotide comparison and phylogenetic tree depending on nucleotide suggest that Gou3 strain is a new subtype from other SEO viruses.
Keywords:ywords:  Hantavirus  Gou3 strain  M segment  Sequence analysis  Phylogenetic tree
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