树鼩实验感染基孔肯雅病毒的研究

选用3株基孔肯雅病毒人工感染成年树鼩,进行了病毒血症、抗体动态变化、内脏组织病理改变和病毒在宿主体内定位的研究。结果表明,感染树鼩能产生2～6天的病毒血症。血凝抑制(Hi)抗体第6天产生,第30～50天达高峰:中和(NT)抗体在第10天产生,第30～40天达高峰,二者相关性非常显著(P<0.01)。补体结合(CF)抗体第14天产生,第40～50天为高峰,以后逐渐下降。第8～12天能在其脑、肺、肝、脾和肾等组织查到病毒,经病理检查这些内脏组织呈炎性改变和出血倾向,表明该病毒能侵袭树鼩各主要脏器。试验认为树鼩对基孔肯雅病毒敏感。

STUDY ON THE EXPERIMENTAL INFECTION OF TREE SHREW WITH CHIKUNGUNYA VIRUS

Adult tree shrews ( Tupaia bslangeri chinensis ) have been tested by inoculation with three strains of Chikungunya ( CHIK ) virus isolated from Yunnan, China and Africa, for their susceptibility. These animals showed viremia for 2-6 days after injection. Specific hemagglutination inhibition ( HI ) antibody appeared on the 6th day following infection and reached peak value on the 30th to 50th day. Neutralization antibody response occured 10 days after infection and reached peak value on the 30-40th day, coexisted with HI antibody. Complement fixation antibody response occured on the 14th day and reached peak value on the 40-50th day after infection. CHIK viruses was detected in the brain, lung, liver, kidney and spleen tissues 4-12 days after infection and disapeared on the 14th day. Pathological changes consisting of inflammation and hemorrhage occurred in the organs of these animals. The results indicate that tree shrew is a susceptible animal to Chikungunya virus, and may be used for studies of CHIK virus.