| 一种快速有效的丙型肝炎病毒cDNA克隆的筛选和鉴定方法 |
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| 引用本文： | 汤权,杨永平.一种快速有效的丙型肝炎病毒cDNA克隆的筛选和鉴定方法[J].病毒学报,1993,9(3):269-273. |
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| 作者姓名： | 汤权 杨永平 |
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| 作者单位： | 江苏盐城市卫生防疫站，中国预防医学科学院病毒学研究所，中国预防医学科学院病毒学研究所，中国预防医学科学院病毒学研究所 224002 进修生，北京，北京，北京 |
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| 摘 要： |
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| 关 键 词： | 原位杂交 探针 丙型肝炎病毒 |
A NEW METHOD FOR RAPID AND EFFICENT SCREENING AND DETERMINIATION OF HCV-cDNA CLONING |
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| Tang Quan Yang Yongping Xia Ningshao Liu Chongbai.A NEW METHOD FOR RAPID AND EFFICENT SCREENING AND DETERMINIATION OF HCV-cDNA CLONING[J].Chinese Journal of Virology,1993,9(3):269-273. |
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| Authors: | Tang Quan Yang Yongping Xia Ningshao Liu Chongbai |
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| Abstract: | During HCV cDNA cloning, cDNA PCR products were cloned into Sma I site of vector pGEM-3Zf,and then specific recombinants were scre-ened by clony hybridization using digoxigenin cDNA probes which were labelled in PCR. Our work demomstrated that this method was highly specific and valuable for the process of cDNA cloning, especially for small amount of cDNA PCR product. Also modified method was more rapid and simpler than others. With this method, we had been successful in the cloning and expression of two regions (C & NS3 genes ) of HCV genome. |
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| Keywords: | In situ hybridization Degoxigenin-labelled probe Hepatitis C virus |
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