不同细胞系对棉铃虫核型多角体病毒复制的受纳性研究

PERMISSIVENESS OF DIFFERENT CELL LINES TO THE REPLICATION OF HELIOTHIS ARMIGERA NUC-LEAR POLYHEDROSIS VIRUS (SINGLY EMBEDDED)

Three different cell lines were infected with Heliothis armigera nuclear polyhedrosis virus ( singly embeded ) ( HaSNPV ) to investigate their per-missiveness for HaSNPV replication. These cell lines are, H. armigera cell line SFE-HA-8212 ( HA-8212 ), Spodoptera frugiperda cell line ( Sf ) , and Chinese hamster ovarian cell line(CHO). The results are as follows. HA-8212 is a permissive cell line that exhitits cytopathic effects typical of NPV infection; formation and release of virulent virus particles by infected cells; synthesis of virai antigens detected by HaSNPV polyclonal an-tibodies and monoclonal antibody MAD7; production of infected cell spe-cific proteins ( ICSPs ); synthesis of virus specific nucleic acid detected by molecular hybridization. CHO cell line is non-permissive and is not af-fected. Sf cell line is semipermissive. Sf celis are disintegrated after HaSNPV infection and synthesis of virus specific nucleic acid is detected at certain stage after HaSNPV infection. Nevertheless, there is no production of virus particles and ICSPs. The practical implications of these results were discussed.