抗体捕捉ELISA法在诊断柯萨奇A16病毒感染中的应用

APPLICATION OF IgM CAPTURE ELISA FOR DIAGNOSIS OF COXSACKIE AI6 VIRUS INFECTION

Using the Coxsackie Al6 virus antigens extracted from the muscles of infected suckling mice, we developed an IgM capture ELISA test for the detection of Coxsackie Al6 virus infection. 27 cases with single sera from hand-foot-mouth disease ( HFMD ) and 16 cases with paired sera from HFMD were tested. Of the 16 cases with paried sera, 13 were CAl6-IgM positive by MacEI.ISA and 12 had IgG 4-fold rise by indirect ELISA. Among the 12 cases with IgG 4-fold rise, the IgM antibody detection rate was 91.67%(11/12) . The specific IgM antibody was detected on second day after onset of the illness. About 80% of the patients were IgM-posi-tive on days 2-4.All the patients were IgM-positive on day 5-25,and then the IgM antibody decreased. 32 day after the illness, no IgM-positive cases were detected. The MacELISA for the detection of CAl6-IgM is a rapid, sensitive and simple method for speific clinical diagnosis.