甲1(H1N1)和甲3(H3N2)型流感病毒在小鼠体内诱导细胞免疫反应的研究

比较了甲型流感病毒A/HK/123/77(H1N1)和A/Qld/6/72(H3N2)及其具有相应表面抗原的两个冷适应基因重分配株(Cold-Adapted Reassortant)的子代毒株CR35和CR6,在小鼠体内诱导初次细胞毒性T细胞(简称Tc)反应的能力。静脉注射相同剂量病毒后第6天,甲3型A/QLd和CR6所诱导的脾细胞Tc活性均比甲1型A/HK和CR35者高100倍。编码内部蛋白的基因相同而表面抗原的基因不同的CR6与CR35所诱导的Tc活性也不同,说明了表面抗原决定着初次Tc反应的强度。用无关的流感病毒A/SW/72(H6N5)攻击已用低剂量(10~4EID_(?))病毒致敏的小鼠表明,对A/Qld和CR6致敏者所诱导的第二次Tc活性,比A/HK和CR3S致敏者的稍高;但对用大剂量病毒致敏者,则均能产生同样好的第二次Tc活性。对Tc识别感染靶细胞上的病毒成份进行了讨论。

CELL MEDIATED IMMUNE RESPONSE TO H1N1 AND H3N2 INFLUENZA VIRUS IN MICE

Two strains of influenza A viruses, A/Hong Kong/123/77 ( A/HK ) ( HlNl )and A/Queensland/6/72 ( A/Qld ) ( H3N2 )and the two cold-adap-ted(ca ) reassortants which posses the surface antigens of these strains, CR35 and CR6 respectively, were compared for thier ability to induce primary cyto-toxic T cell(Tc ) responses in mice.When similar doses ( IO or IO3 HAU ) of each virus were injected i.v.into mice and the spleens tested for Tc activity 6 days later, both A/Qld and CR6 were about IOO-fold higher in inducing a primary Tc responses than A/HK or CR35, The use of the two reassortant viruses which contain gene coding for identical internal proteins shows conclusively that tha surface protiens determine the magnitude of the primary Tc response under these circumstances, Whether the hemagglutinin and neuramini-dase are equally important remains to be determined.These strains were also tested for thier ability to sensitize mice for a secondary Tc response challenged with a distantly related A strains virus A/Shearwater (A/SW)/77 ( H6N5 ) .If mice were primed with a low dose ( IO4 E-ID50 )of these viruses ,A/Qld and CR6 were more effective than A/HK or CR35 at sensitizing for a secondary Tc response when challenged with A/S-W, but if larger doses were given either i.n. (IO6 EID50 ) or i.v. (IO3HAU) all viruses sensitized the mice equally well, despite the fact that A/SW gave a poor primary Tc response in mice.We also disscussed that probably Tc cells recognized internal virus determinants on the membrane of infected target cells.