新疆幼畜和人非典型轮状病毒的调查和鉴定

INVESTIGATION AND IDENTIFICATION OF ATYPICALROTAVIRUS FROM ANIMALS AND HUMAN

214 stool samples collected from animals and men during the last ten years (1984-1994)were examined for the presence of typical and atypical rotavirus by PAGE.56/1226 lamb and did,144/510 piglet,4/110 calf,2/108 chicken,26/68 infant and 46/113 the neonatal withoutdiarrhea were shown to contain group A rotavirus.No group A rotavirus was detectedin human adult and camel stool samples.16/1126 lamb and kid,10/510 piglet were shown tocontain group B rotavirus and no group B rotavirus was detected in other animals and men.GCRV was only detected in piglet(22/510).GDRV was only in chicken(2/108).GERV like wasonly in piglet(1/510). Nine strains of atypical rotavirus(ie.KB-63,LB-60,MPB-1,MPB-2,SPB-3,SPB-4,SPC-11, ZPB-12,piglet group E-like rotavirus)which belong to different rotavirus groupswere inoculated into neonatal animals deprived of clostrum.All animals developed acutewatery diarrhea and shed virus during 12-16 hours postinoculation.Group antigen of the nine strains as mentioned above and one strain of ADRV wereexamined with group A-ELISA,group B-ELISA and countercurrent immune electrophoresis(CIE). Dot hybridization of these strains were also carried out with photobiotin-labelednucleic acid probes for group A and group B rotavirus.The results indicated that ADRV,LB-60,KB-63,MPB-1,MPB-2,SPB-3,SPB-4 and the pigler group E-like rotaviruscontained group B rotavirus antigen only.They hybridiaed only with group B rotavirus nucleicacid probe.Group C rotavirus (i,e.SPC-11,ZPC-12) contained neithfer group A and group B rotavirus antigen nor hybridized with group A or group B nucleic acid probes.