用狂犬病毒单克隆抗体2E9在小鼠引发狂犬病早期死亡综合征的实验研究

STUDY ON THE "EARLY DEATH" SYNDROME IN MICE BY USING MONOCLONAL ANTIBODIES TO RABIES VIRUS

Monoclonal antibodies, 9E6 and 2E9 are directed against two different epitopes on the surface of 61kD glycoprotein of rabies virus ( RV ) . Passively transferred 2E9 to mice before or after rabies virus challenge could cause early death of some challenged mice. Also, its occurence depends on the concentration of McAb and the administration time.At 5 days before challenge with RV, 2E9 ( 1 : 300 ) induced early death syndrome. On 5 and 2 days before challenge or 2 days after challenge, 2E9 ( 1 : 30 ) consistently induced a early death syndrome. However, under the same condition 9E6 did not induce this phenomenon, but produced a protective effect against rabies virus. The results suggested that the occurrence of early death is related to a given epitope on the antigen and is induced by the antibody which reactes with this epitope. The concentration of the passively transferred antibody and the administration time are also important factors.