首页 | 本学科首页   官方微博 | 高级检索  
   检索      

鸡源和鹅源新城疫病毒在宿主细胞上的蚀斑形成特性及形态发生学比较
引用本文:冯新,宋战昀,邹啸环,韩文瑜,丁壮.鸡源和鹅源新城疫病毒在宿主细胞上的蚀斑形成特性及形态发生学比较[J].病毒学报,2010,26(1):58-64.
作者姓名:冯新  宋战昀  邹啸环  韩文瑜  丁壮
作者单位:吉林大学,畜牧兽医学院预防兽医学国家重点学科,吉林,长春,130062;吉林出入境检验检疫局,吉林,长春,130062;军事医学科学院,军事兽医研究所,吉林,长春,130062
基金项目:国家自然科学基金资助项目(30771606)
摘    要:通过蚀斑形成试验比较鸡源(F48E9株)和鹅源(NA-1株)新城疫病毒在不同细胞的蚀斑形成能力,结果显示F48E9、NA-1两种病毒在Vero细胞、鸡胚成纤维细胞(CEF)和鹅胚成纤维细胞(GEF)上蚀斑形成能力存在明显的差异,在GEF上NA-1株的蚀斑形成能力强于F48E9株,而在CEF上弱于F48E9株,在Vero细胞上NA-1株的蚀斑形成能力稍强于F48E9株,表明病毒蚀斑形成特性与宿主种属特性一致。通过电镜观察两株病毒以相同感染量感染Vero细胞后的病毒形态发生过程。NA-1株和F48E9株病毒在Vero细胞中不同时段的形态发生的进程上存在区别,无论从出芽时间还是出芽后病毒囊膜的完整性上,NA-1株均优于F48E9株,提示NA-1株病毒对宿主环境的适应力较强。

关 键 词:新城疫病毒  形态发生  病毒蚀斑  透射电子显微镜

Comparison of Plaque-Forming Characteristics and Morphogenetic Changes of Newcastle Disease Virus Isolated from Chickens and Geese on Host Cells
FENG Xin,SONG Zhan-yun,ZOU Xiao-huan,HAN Wen-yu,DING Zhuang.Comparison of Plaque-Forming Characteristics and Morphogenetic Changes of Newcastle Disease Virus Isolated from Chickens and Geese on Host Cells[J].Chinese Journal of Virology,2010,26(1):58-64.
Authors:FENG Xin  SONG Zhan-yun  ZOU Xiao-huan  HAN Wen-yu  DING Zhuang
Institution:1.Animal Science and Veterinary College of Jilin University;Changchun 130062;China;2.Jilin Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China;3.Institute of Military Veterinary;Academy of Military Medical Sciences;China
Abstract:The plaque-forming characteristics of Newcastle disease viruses of chickens and geese source were compared on various cells.The result showed that there were obvious differences of plaque formation between F48E9 and NA-1 on Vero cells,chicken embryo fibroblast cells(CEF) and goose embryo fibroblast cells(GEF).The plaque-forming ability of NA-1 was higher than F48E9 on GEF,but lower than F48E9 on CEF.On Vero cells,the plaque-forming ability of NA-1 was slightly stronger than F48E9.It demonstrated that the pl...
Keywords:Newcastle disease  morphogenesis  virus plaque  transmission electron microscope  
本文献已被 CNKI 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号