HIV—Pol基因的套式PCR检测

DETECTION OF HIV-1 POL GENE BY NESTED POLYMERASE CHAIN REACTION

group of nested primers corresponding to HIV-1 MN strain Pol gene was designed and synthesized .Peripheral blood mononuclear cell lysates were first amplified with outer primers.Its products further amplified with the inner nested primers.The final amplified products were detected by agarose gel electrophoresis. The sensitivity of nested PCR is 100 to 1000 times higher than the standard PCR and can detect 10 molecules of template DNA.Our data showed that the HIV-1 Pol gene detection by nested PCR is valuable in early diagnosis ,confirmation of HIV 1 infecton and HIV-1 isolation.It is a simple gene detecting method with high specificity and sensitivity.