甜菜坏死黄脉病毒内蒙分离物自然缺失突变体缺失区域的定位分析

以甜菜坏死黄脉病毒内蒙分离物（ＢＮＹＶＶ ＮＭ）总ＲＮＡ为模板，经ＲＴ－ＰＣＲ扩增，分别获得ＲＮＡ２、ＲＮＡ３和ＲＮＡ４自然缺失突变体ｃＤＮＡ克隆。序列分析结果表明，ＲＮＡ２自然缺失突变体在７５ｋＤ通读蛋白编码区Ｃ端缺失３４８个核苷酸（缺失位置ｎｔ１４８８￣ｎｔ１８３５）。ＲＮＡ３在其２５ｋＤ蛋白编码区内缺失３６０个核苷酸（缺失位置ｎｔ７２９￣ｎｔ１０８８）。ＲＮＡ４的自然缺失区域位于３１ｋＤ蛋白

Location of the Deleted Sequence of Spontaneously Deleted RNAs of Beet Necrotic Yellow Vein Virus NM lsolate

Spontaneously deleted RNA2, RNA3 and RNA4 cDNA clones were obtained respectively by cloning the RT PCR products of beet necrotic yellow vein virus NM isolate from Inner Mongolia into pGEM 7Zf(+). The sequence analysis showed that the spontaneously deleted RNA2 has 348 nucleotide deletion (nt1,488 nt1,835) within its C terminal region of the 75 kD readthrough protein gene. The RNA3 has 360 nucleotide deletion(nt729 nt1,088) within its 25kD protein ORF region. The RNA4 has 402 nucleotide deletion(nt702 nt1,103) within its 31kD protein ORF region, which encoded a protein consisting of 148 amino acids. The spontaneously deleted regions in the RNAs of BNYVV NM isolate were much more similar to the German isolate G1 and Japanese isolate S. The possible mechanism of spontaneous deletion of the virus RNAs and its application for control of the disease were discussed in this paper as well.