中国蝙蝠腺病毒遗传多样性研究

近期的研究表明蝙蝠可以携带腺病毒。为了进一步了解我国蝙蝠腺病毒的分布状况和遗传多样性特征,本研究采集了北京市、湖南省、江西省、云南省、贵州省和海南省六个省份的共11种蝙蝠的咽拭和肛拭样本,使用套式PCR方法进行蝙蝠腺病毒检测。对阳性结果通过克隆测序进行遗传多态性分析,并采用基于氨基酸相似性的核酸序列数据进行分子进化研究。结果表明,我国约20%的蝠种携带腺病毒,特别是大足鼠耳蝠可能普遍携带蝙蝠腺病毒。并且在贵州省南蝠样本中同时检测出两种不同的蝙蝠腺病毒。总体而言,蝙蝠腺病毒DNA聚合酶保守区核酸和蛋白序列的平均相似性分别只有66.6%和74.7%。蝠种和地域上的巨大差异可能导致了蝙蝠腺病毒的适应性进化,而形成了显著的遗传多样性。

Genetic Diversity of Adenoviruses in Bats of China

Adenovirus remains a significant threat to public health.Recent studies showed that bats can harbor diverse adenoviruses.To further investigate the distribution and genetic diversity of bat adenoviruses in China,we collected throat and anal swab samples of 11 bat species from 6 provinces of China,including Beijing,Hunan,Jiangxi,Yunnan,Guizhou and Hainan.Nested PCR was used to identify potential bat adenoviruses from the samples,and positive results were cloned and sequenced for genetic diversity study.In addition,nucleotide sequence alignments based on corresponding amino acid sequence similarities were used for phylogenetic analyses.Our results showed that about 20% of bat species in China are positive to adenoviruses,and Myotis ricketti is likely to be the most important host of bat adenoviruses in all locations.Moreover,we identified two diverse sequences of bat adenoviruses from the same sample of Ia io in Guizhou province of China.In general,the average nucleotide and amino acid sequence similarities of the conserved region of DNA polymerases of bat adenoviruses are 66.6% and 74.7%,respectively.The differences between bat species and their residences environments may have driven the adaptive evolution of the viruses,leading to the genetic diversity of the bat adenoviruses.