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猪伪狂犬病毒鲁A株TK基因的序列测定及其结构功能与进化分析
引用本文:范伟兴,张雪莲,赵宏坤,陈溥言.猪伪狂犬病毒鲁A株TK基因的序列测定及其结构功能与进化分析[J].病毒学报,2002,18(3):249-258.
作者姓名:范伟兴  张雪莲  赵宏坤  陈溥言
作者单位:1. 南京农业大学动物医学院,南京,210095;山东农业大学,动物科技学院,泰安,271018;2. 南京农业大学动物医学院,南京,210095;3. 山东农业大学,动物科技学院,泰安,271018
摘    要:对猪伪狂犬病毒鲁A株(PRV LA株)TK基因进行了克隆和序列测定,并分析比较了该序列与PRV NIA-3株、Ea株、SH以及HSV-1和VZV的同源性,结果表明:在全长1048bp的DNA序列中,包括着一个963bp的开放阅读框(ORF),可编码320个氯基酸组成的多肽;在整个TK基因的ORF内,PRV LA株与PRV NIA-3株、PRV Ea株、PRV SH株、HSV-1、VZV的TK基因比较,核苷酸的同源性分别为98.9%、99.5%、99.3%、36.4%、39.1%,氨基酸的同源性分别为98.4%、99.7%、98.7%、36.6%、37.2%.PRV LA株TK具有疱疹病毒胸苷激酶催化结构域的保守氨基酸共有序列和亚结构域特征序列.将PRV-LA TK、人类和小鼠的胸苷酸激酶、人类脱氧胞苷激酶、人类腺苷酸激酶的对应于这两个亚结构域的氨基酸用DNA Star分析的进化树表明,疱疹病毒的TK与人类和小鼠的胸苷酸激酶的亲缘关系比与人类脱氧胞嘧啶激酶的亲缘关系更近,因此疱疹病毒的TK基因在进化上可能起源于宿主细胞的胸苷酸激酶基因.

关 键 词:伪狂犬病毒LA株  胸苷激酶(TK)  核苷酸序列  氨基酸序列  疱疹病毒TK来源
文章编号:1000-8721(2002)03-0249-010

Cloning,Sequencing and Structural Evolution Analysis of the Thymidine Kinase Gene of a Wild Chinese PRV LA Strain
FAN Wei xing ,ZHANG Xue lian ,ZHAO Hong kun ,CHEN Pu yan.Cloning,Sequencing and Structural Evolution Analysis of the Thymidine Kinase Gene of a Wild Chinese PRV LA Strain[J].Chinese Journal of Virology,2002,18(3):249-258.
Authors:FAN Wei xing    ZHANG Xue lian  ZHAO Hong kun  CHEN Pu yan
Institution:FAN Wei xing 1,2,ZHANG Xue lian 1,ZHAO Hong kun 2,CHEN Pu yan 1
Abstract:The thymidine kinase(TK)gene in the unique long region of the wild Chinese RPV LA strain was amplified by polymerase chain reaction(PCR).The PCR product was ligated to pUC19 plasmid vector and the nucleotides of the TK gene was sequenced.An analysis of the nucleotide sequence and its deduced amino acid sequence were performed with computer programs.The results revealed that the region of DNA sequenced is 1048 base pairs and it has one ORF of 963 base pairs to have the potential to encode a protein of 320 amino acids.The TK gene of PRV LA strain shares highly conserved nucleotides with PRV NIA-3 strain,Ea strain and SH strain.The homology of the nucleotide sequence of PRV LA strain with PRV NIA-3 strain,Ea strain,SH strain,HSV-1,VZV is 98.9%,99.5%,99.3%,36.4% and 39.1%,respectively;and the homology of deduced amino acids among them is 98.4%,99.7%,98.7%,36.6% and 37 2%,respectively The amino acids of PRV TK contain the conserved ATP-binding motifs(-GXXGXGKT-)and the conserved necleosite-binding motifs(-DRH-) The similarity of the two conserved bindings among PRV-LA TK and homo sapiens thymidylate kinase,mus musculus thymidylate kinase,homo sapiens deoxycytidine kinase and homo sapiens adenylate kinase indicated that the herpes virus TK was derived from the infected-cell encoded thimidylate kinase
Keywords:wild  chinese  pseudorabies  virus  LA  strain  thymidine  kinase  nucleotide  sequence  amino  acid  sequence  origin  of  herpes  virus  TK
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