| 采用病毒受体基因转移技术建立EB病毒细胞感染模型 |
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| 引用本文： | 纪志武 Takad.,K.采用病毒受体基因转移技术建立EB病毒细胞感染模型[J].病毒学报,1994,10(2):154-158. |
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| 作者姓名： | 纪志武 Takad. K |
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| 作者单位： | 中国预防医学科学院病毒学研究所，日本国山口大学医学部寄生虫学与微生物学教室 |
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| 摘 要： |
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| 关 键 词： | 细胞转染 疱疹病毒 受体 基因转移 |
ESTABLISHMENT OF CELL INFECTION MODEL OF EPSTEIN-BARR VIRUS BY TRASFECTION WITH THE RECEPTOR GENE |
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| Ji Zhiwu K.Takada Li Baomin,Zeng Yi.ESTABLISHMENT OF CELL INFECTION MODEL OF EPSTEIN-BARR VIRUS BY TRASFECTION WITH THE RECEPTOR GENE[J].Chinese Journal of Virology,1994,10(2):154-158. |
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| Authors: | Ji Zhiwu KTakada Li Baomin Zeng Yi |
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| Institution: | Ji Zhiwu K.Takada* Li Baomin Zeng Yi |
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| Abstract: | he normal host range of EB virus (EBV)is limited to primate B lymphocytesand cer-tain epithelial cells that express the CR2/EB virus receptor.An eukaryotic expression plasmidpSG5-CR2 encoding CR2 gene cDNA sequence was successfully constructed ,and the CR2 ex-pressed well in Wish and CNE3 cells.The CR2 positive cell is about 5.Both wish and CNE3 cells were infected by B95-8 strain of EB virus after the plasmid pSG5-CR2transfection.Curing the day 2-25after the EB virus infection,EBV related antigens includingEBNAl,early and capsid antigens were detected in cells by immunological assay.The infection of both Wish and CNE3 cells through expression of recombinant human CR2would facilitate the analysis of cellular factors that influence the viral growth and the mechanism by which EB virus infection induces nasopharyngeal carcinoma in human. |
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| Keywords: | Epstein-Barr virus CR2 gene Cell transfection Plasmid pSG5-CR2 |
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