我国常见乙型肝炎病毒基因型与基本核心启动子及前C区突变关系的初步研究

收集81份HBV DNA阳性血清标本,经PCR扩增和序列测定确定其中有50份属于基因型C,31份属于基因型B;C基因型的基本核心启动子BCP T1762/A1764的突变率(38%)明显高于B基因型(12.9%,P<0.05);前C区A1896的突变在B、C两基因型间无显著性差异,B基因型为9.7%,C基因型为12%,P>0.05;HBeAg的表达与否与BCP双突变或前C区A1896突变均无明显相关性。经定量PCR检测证明,HBeAg阳性组中的HBV DNA含量明显高于抗-HBe阳性组,P<0.05。组内BCP双突变株和野生株及前C1896突变株和野生株的HBV DNA含量无显著性差异。

Initial Study on Relationship between the Common HBV Genotypes in China and the Basic Core Promoter or Precore Mutations

81 serum specimens positive for HBV DNA were collected from Shanxi Province.After sequence amplification and analysis of the core gene,50 specimens were found belonging to genotype C,and the others were genotype B.The double mutation in BCP(T1762/A1764) was obviously more frequent in genotype C(38%) than that in genotype B patients(12.9%,P<0.05).However,there was no significant difference of the PreC(A1896) mutation between genotypes B(9.7%) and C(12%,P>0.05).No relationship between expression of HBeAg and double-mutation of BCP(T1762/A1764) or PreC(A1896) mutation was found.The result of qPCR was that the Ct value was significantly higher in HBeAg positive group than that in anti-HBe positive group(P<0.05).There was no significant difference of HBV DNA content between BCP double mutant type and the wild type in HBeAg and anti-HBe groups.The same comparative result was obtained in preC A1896 mutant and the wild type.