| 人乳头瘤病毒16型-L1真核表达质粒的构建和表达以及实验动物对裸DNA的免疫反应 |
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| 引用本文： | 孙向乐,司履生,曹瓒孙,王一理,刘天菊,郭建芬.人乳头瘤病毒16型-L1真核表达质粒的构建和表达以及实验动物对裸DNA的免疫反应[J].病毒学报,1999(3). |
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| 作者姓名： | 孙向乐 司履生 曹瓒孙 王一理 刘天菊 郭建芬 |
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| 作者单位： | 西安医科大学免疫病理研究室!西安710061 |
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| 摘 要： |
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| 关 键 词： | 人乳头瘤病毒16型 DNA疫苗 宫颈癌 |
THE ESTABLISHMENT OF HPV16-L1 EUKARYOTIC EXPRESSION SYSTEM AND ITS IMMUNOGENICITY STUDY |
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| Sun Xiangle Si Lusheng Cao Zansun,Wang Yili Liu Tianju Guo Jianfen.THE ESTABLISHMENT OF HPV16-L1 EUKARYOTIC EXPRESSION SYSTEM AND ITS IMMUNOGENICITY STUDY[J].Chinese Journal of Virology,1999(3). |
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| Authors: | Sun Xiangle Si Lusheng Cao Zansun Wang Yili Liu Tianju Guo Jianfen |
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| Institution: | Sun Xiangle Si Lusheng Cao Zansun * Wang Yili Liu Tianju Guo Jianfen |
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| Abstract: | Recombinant plasmid pcDNA-L1 was constructed by inserting HPV16-L1 gene fragment into eukaryotic expression plasmid pcDNA3.1. pcDNA-L1 was transfected into mammalian cells Cos-7 and the expression of HPV16-L1 protein was testified by immunohistochemistry(IHC). Then the recombinant plasmid was directly injected into the quadriceps muscle of BALB/c mice. Antibodies against HPV16-L1 in the immunized mice were positively detected by immunodot and IHC at 28 and 41 days after the last immunization. |
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| Keywords: | ywords: HPV16 DNA vaccine Cervical cancer |
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