深圳市新甲型H1N1流感病毒感染病例的血清学及分子特点分析

本研究通过对深圳市4名甲流重症患者的血清抗体及其所感染的新甲型H1N1流感病毒的抗原性和分子特点的分析,发现这些患者在感染后短期内产生的血清中和抗体滴度均不超过1:20,不能起到有效的保护作用;交叉血凝抑制实验的结果显示新H1N1病毒与季节性H1N1和H3N2流感病毒无任何交叉反应,抗原性差异很大,而患者所感染的病毒与标准株的抗原性则没有太大差异;分子特点的分析表明新H1N1病毒进入人群后依然属于经典的猪流感亚系,4名重症患者感染的病毒不具备高致病性流感病毒的遗传特点,几个氨基酸位点的变异没有影响病毒的毒力和致病性,只有一株毒株的NA蛋白发生了His275Tyr的突变,产生了对达菲等神经氨酸酶抑制剂的耐药性。

Analyses of Serological and Genetic Characteristics on Novel H1N1 Influenza A Virus from the Infected Patient in Shenzhen

Analysis of serological and genetic characteristics on 2009 swine-origin influenza A(H1N1) virus(S-OIV) isolated from four patients with severe disease in Shenzhen were performed.Microneutralization assay showed that the neutralizing antibody titers of the infected patients did not exceed 1:20 in a short term post infection,which could not neutralize the viruses efficiently.Hemagglutination inhibition(HI) tests confirmed that the antigenicity of S-OIV from the patients was distinct from the seasonal influen...