| HPV-2E2蛋白(A338V)突变体对E2蛋白DNA结合能力增强的研究 |
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| 引用本文： | 潘明明,高晨,李晓丽,巩含实,石琦,袁育康,范桂香,董小平.HPV-2E2蛋白(A338V)突变体对E2蛋白DNA结合能力增强的研究[J].病毒学报,2010,26(3). |
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| 作者姓名： | 潘明明 高晨 李晓丽 巩含实 石琦 袁育康 范桂香 董小平 |
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| 作者单位： | 西安交通大学,医学院,西安,710061;中国疾病预防控制中心病毒病预防控制所传染病预防控制国家重点实验室,北京,100052;中国疾病预防控制中心病毒病预防控制所传染病预防控制国家重点实验室,北京,100052;西安交通大学,医学院,西安,710061 |
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| 基金项目： | 传染病预防控制国家重点实验室基金(2008SKLID202); 传染病重大专项(2009ZX10004-101) |
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| 摘 要： | HPV-2是引起皮肤寻常疣的常见HPV型别,病毒E2蛋白可抑制病毒早期启动子的活性。我们曾经报道来自一例巨大寻常疣患者的HPV-2突变E2蛋白对病毒早期启动子活性的抑制作用明显减弱,该E2蛋白在其C末端的DNA结合区域带有A338V的点突变。本研究利用原核表达系统表达纯化了突变E2(A338V)和HPV-2原毒株的羧基端和全长蛋白。电泳迁移率实验结果显示,E2蛋白可与带有E2蛋白特异性结合位点的寡核苷酸探针形成复合物,突变E2蛋白比原毒株E2蛋白的DNA结合能力强。这提示DNA结合能力的增强可能为E2蛋白对病毒启动子活性影响的分子基础,与患者出现罕见巨大寻常疣这一临床表型关联。
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| 关 键 词： | HPV-2 E2蛋白 DNA结合能力 |
The Enhancement of DNA Binding Ability of a Mutated E2(A338V) Protein of HPV-2 |
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| PAN Ming-ming,GAO Chen,LI Xiao-li,GONG Han-shi,SHI Qi,YUAN Yu-kang,FAN Gui-xiang,DONG Xiao-ping.The Enhancement of DNA Binding Ability of a Mutated E2(A338V) Protein of HPV-2[J].Chinese Journal of Virology,2010,26(3). |
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| Authors: | PAN Ming-ming GAO Chen LI Xiao-li GONG Han-shi SHI Qi YUAN Yu-kang FAN Gui-xiang DONG Xiao-ping |
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| Institution: | PAN Ming-ming1,2,GAO Chen2,LI Xiao-li2,GONG Han-shi1,SHI Qi2,YUAN Yu-kang1,FAN Gui-xiang1,DONG Xiao-ping2 (1.School of Medicine,Xi'an Jiao-Tong University,Xi'an 710061,China,2.State Key Laboratory for Infectious Disease Prevention and Control,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 100052,China) |
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| Abstract: | HPV-2 is a very common type of HPV which causes common warts. The E2 protein of virus can repress the activity of the viral early promoter through binding to the specific binding sites in viral LCR. Previously we reported that the repression of a mutated E2 protein of HPV-2 isolated from a patient with huge common wart on the viral early promoter was obviously decreased,and A338V mutation located at the C terminal DNA binding region of E2 protein. In this study,we expressed and purified the recombinant muta... |
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| Keywords: | HPV-2 E2 DNA binding ability |
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