| 中国柯萨奇病毒B5的全基因组测序及其序列分析 |
| |
| 引用本文： | 胡永峰,赵丽娜,董杰,吴志强,杜江,薛颖,杨帆.中国柯萨奇病毒B5的全基因组测序及其序列分析[J].病毒学报,2010,26(4). |
| |
| 作者姓名： | 胡永峰 赵丽娜 董杰 吴志强 杜江 薛颖 杨帆 |
| |
| 作者单位： | 中国疾病预防控制中心,病毒病预防控制所,病毒基因工程国家重点实验室,北京,100052;中国医学科学院病原生物学研究所,北京,100176;华北煤炭医学院生物科学系,唐山,063000;中国医学科学院病原生物学研究所,北京,100176 |
| |
| 摘 要： | 本文对我国首株与手足口病相关的柯萨奇病毒B5(01/CVB5/SD/CHN/09,CVB5/09)进行了基因组测序并与现有的相关序列进行了比较和进化分析。CVB5/09基因组长7399nt,共编码氨基酸2185aa,与现有的CVB5基因组核酸序列相似性在80.6%～85.3%之间,氨基酸序列相似性在96.1%～96.9%。进化分析发现,利用不同的基因组片段P1、P2和P3区构建的进化树中,CVB5/09分别处在不同的进化分支上,不同基因组片段有着不同的进化速率。Simplot相似性分析没有发现基因组有明显的重组发生。本文完成了我国第1株柯萨奇病毒B5全基因组序列的测定,通过与其它相关病毒的比较分析深入了解其遗传特征,以期为手足口病的流行病学调查和预防控制提供有价值的信息。
|
| 关 键 词： | 手足口病 柯萨奇病毒B5 基因组测序 序列分析 |
Complete Genome Sequence of Coxsackievirus B5(CVB5/09)Strain Isolated in China and Its Phylogenetic Analysis |
| |
| HU Yong-feng,ZHAO Li-na,DONG Jie,WU Zhi-qiang,DU Jiang,XUE Ying,YANG Fan.Complete Genome Sequence of Coxsackievirus B5(CVB5/09)Strain Isolated in China and Its Phylogenetic Analysis[J].Chinese Journal of Virology,2010,26(4). |
| |
| Authors: | HU Yong-feng ZHAO Li-na DONG Jie WU Zhi-qiang DU Jiang XUE Ying YANG Fan |
| |
| Institution: | HU Yong-feng1,ZHAO Li-na2,3,DONG Jie2,WU Zhi-qiang2,DU Jiang2,XUE Ying2,YANG Fan2(1.State Key Laboratory for Molecular Virology and Genetic Engineering,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 100052,China,2.Institute of Pathogen Biology,Chinese Academy of Medical Sciences,Beijing 100176,3.Department of Biological Sciences of North China Coal Medical College,Tangshan,063000 China) |
| |
| Abstract: | An outbreak of hand foot and mouth disease occurred in Shang dong,China in 2009.Almost 20% of patient's swabs was positive for Coxsackie virus B5(CVB5)identified by RT-PCR and sequencing.It was suggested that CVB5 may be another important pathogen for HFMD.Fifteen pairs of overlapping primers were designed and the genome sequence was sequenced.The genome of CVB5 was 7 399 nt in length,coding for 2 185aa.The genome displayed 80.6%-85.3% nucleotide sequence identity and 96.1%-96.9% amino acid sequence identit... |
| |
| Keywords: | hand foot and mouth disease genome sequence phylogenetic analysis |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
