| 中国牛病毒性腹泻病毒JZ05-1分离株全基因测序及分析 |
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| 引用本文： | 李庆超,苗利光,李海涛,刘艳环,张广雷,肖佳美.中国牛病毒性腹泻病毒JZ05-1分离株全基因测序及分析[J].病毒学报,2010,26(3). |
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| 作者姓名： | 李庆超 苗利光 李海涛 刘艳环 张广雷 肖佳美 |
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| 作者单位： | 中国农业科学院,吉林特产研究所,吉林市,132109;江苏科技大学,镇江,212018 |
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| 基金项目： | 国家科技支撑计划项目;(2006BAD06B06) |
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| 摘 要： | 牛病毒性腹泻病毒为瘟病毒属成员,呈世界性分布并在乳/肉牛业中造成严重经济损失。本研究利用MD-BK细胞增殖一株分离于我国吉林地区的牛源牛病毒性腹泻病毒JZ05-1毒株,观察发现其具有典型的致细胞病变效应。使用逆转录-聚合酶链式反应(RT-PCR)方法分别扩增覆盖基因组全长的八个片段并测序,拼接获得该病毒的全基因组序列长12285核苷酸(nt),GenBank登录号:GQ888686。该病毒基因组具有编码多聚蛋白的一个11694nt可读框,5'非翻译区(UTR)长387nt,3'非翻译区长204nt。分别分析BVDVJZ05-1的5'-UTR序列和全基因组序列,发现该病毒属于BVDV-2a亚型。BVDV-2型多数毒株之间的相似性约为96%,与JZ05-1全基因组序列相似性最高的加拿大p11Q毒株和中国XJ-04毒株的相似性为90%和91%。因此,JZ05-1全基因组序列与BVDV-2型其他毒株的全基因组序列具有较大差异。
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| 关 键 词： | BVDV 全基因组 基因型 相似性 |
Genome Sequencing and Analysis of the Bovine Viral Diarrhea Virus-2 Strain JZ05-1 Isolated in China |
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| LI Qing-chao,MIAO Li-guang,LI Hai-tao,LIU Yan-huan,ZHANG Guang-lei,XIAO Jia-mei.Genome Sequencing and Analysis of the Bovine Viral Diarrhea Virus-2 Strain JZ05-1 Isolated in China[J].Chinese Journal of Virology,2010,26(3). |
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| Authors: | LI Qing-chao MIAO Li-guang LI Hai-tao LIU Yan-huan ZHANG Guang-lei XIAO Jia-mei |
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| Institution: | LI Qing-chao1,MIAO Li-guang1,LI Hai-tao1,LIU Yan-huan1,ZHANG Guang-lei2,XIAO Jia-mei1 (1. Institute of Special Wild Economic Animal and Plant Sciences,Chinese Academy of Agricultural Sciences,Jilin 132109,China,2. Jiangsu University of Science and Technology,Jiangsu 212018,China) |
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| Abstract: | Bovine viral diarrhea virus (BVDV) is a member of the genus Pestivirus,which is a widespread problem for beef and dairy herds,and has given rise to a significant loss in the livestock industry all over the world. The BVDV strain JZ05-1 isolated from cattle in Jilin,China generated cytopathic effect (CPE) in MDBK cells. Eight overlapped gene fragments were amplified by RT-PCR and sequenced,the complete genom sequence of BVDV strain JZ05-1 was assembled. According to the results,the JZ05-1 genome was composed... |
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| Keywords: | BVDV genome sequence genotype similarity |
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