| 新城疫病毒分离株的生物学特性鉴定及F蛋白基因序列分析 |
| |
| 引用本文： | 姚春峰,刘文博,胡顺林,马怀良,薛峰,刘慧谋,刘秀梵.新城疫病毒分离株的生物学特性鉴定及F蛋白基因序列分析[J].病毒学报,2009,25(2):117-124. |
| |
| 作者姓名： | 姚春峰 刘文博 胡顺林 马怀良 薛峰 刘慧谋 刘秀梵 |
| |
| 作者单位： | 扬州大学,农业部畜禽传染病学重点开放实验室,江苏,扬州,225009;扬州大学,农业部畜禽传染病学重点开放实验室,江苏,扬州,225009;扬州大学,农业部畜禽传染病学重点开放实验室,江苏,扬州,225009;扬州大学,农业部畜禽传染病学重点开放实验室,江苏,扬州,225009;扬州大学,农业部畜禽传染病学重点开放实验室,江苏,扬州,225009;扬州大学,农业部畜禽传染病学重点开放实验室,江苏,扬州,225009;扬州大学,农业部畜禽传染病学重点开放实验室,江苏,扬州,225009 |
| |
| 基金项目： | “十一五”国家科技支撑计划(2006BAD06A03);;国家自然科学基金重点项目(30630048);;江苏省教育厅指导计划项目(06KJD230211) |
| |
| 摘 要： | 通过部分生物学特性鉴定、RT-PCR及F基因的序列测定与遗传进化分析,对2005~2006年从我国江苏省和广西省部分地区的发病鸡群和鹅群中分离到的20株新城疫病毒(NDV)进行了研究。各分离株经典毒力测定结果显示:MDT在45.3h~58.2h之间,ICPI在1.61~2.00之间,均为新城疫病毒强毒株特征。血凝解脱及血凝素热稳定性试验显示:各分离株的血凝解脱时间短,血凝素热稳定性较差,符合NDV强毒株的特征。F基因的序列测定表明,分离株之间的核苷酸序列具有79.7%~100%的同源性,与疫苗株LaSota的同源性为78.1%~83.4%;与国内标准强毒株F48E8同源性为80.2%~90.1%。推导其氨基酸序列分析表明,各分离株的F蛋白的裂解位点氨基酸组成为112R-R-Q-R/K-R-F117,具有NDV强毒株特征,与毒力测定结果相符。根据序列所绘制系统进化发生树,表明20株NDV分离株中有18株为基因Ⅶd型,2株为基因Ⅲ型。
|
| 关 键 词： | 新城疫病毒 生物学特性 毒力 F基因 序列分析 |
Biological Characteristics and Sequence Analysis of Fusion Genes of Newcastle Disease Virus Isolates |
| |
| YAO Chun-feng,LIU Wen-bo,HU Shun-lin,MA Huai-liang,XUE Feng,LIU Hui-mou,LIU Xiu-fan.Biological Characteristics and Sequence Analysis of Fusion Genes of Newcastle Disease Virus Isolates[J].Chinese Journal of Virology,2009,25(2):117-124. |
| |
| Authors: | YAO Chun-feng LIU Wen-bo HU Shun-lin MA Huai-liang XUE Feng LIU Hui-mou LIU Xiu-fan |
| |
| Institution: | Key Laboratory of Animal Infectious Diseases of Ministry of Agriculture;Yangzhou University;Yangzhou 225009;China |
| |
| Abstract: | Twenty Newcastle disease virus(NDV) strains were isolated from chickens and geese in the field outbreaks during 2005 and 2006 in some regions of Jiangsu and Guangxi province.Assessment of the virulence by MDT and ICPI,RT-PCR and sequence analysis of fusion protein gene were used to compare the properties of NDV isolates.The results indicated that MDT and ICPI of the isolates were 45.3h~58.2h and 1.61~2.00 respectively,which confirmed that the all NDV isolates were highly virulent.And their hemagglutinin wer... |
| |
| Keywords: | NDV Biological characteristic Virulence Fusion gene Sequence analysis |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
