| 荧光定量PCR技术评价亚甲蓝光化学法病毒灭活效果的研究 |
| |
| 引用本文： | 张博,郑岚,黄宇闻,莫琴,王迅,钱开诚.荧光定量PCR技术评价亚甲蓝光化学法病毒灭活效果的研究[J].病毒学报,2009,25(4):286-290. |
| |
| 作者姓名： | 张博 郑岚 黄宇闻 莫琴 王迅 钱开诚 |
| |
| 作者单位： | 华东师范大学,上海市血液中心,博士研究生培养基地,上海,200051;上海市血液中心,上海,200051 |
| |
| 基金项目： | 上海市医学领先专业重点学科建设项目(05III003);;上海市卫生局课题资助(2007019) |
| |
| 摘 要： | 本研究探讨利用荧光定量PCR技术评价Sindbis病毒经亚甲蓝光化学处理后灭活效果的可行性。研究采用不同光照强度对Sindbis病毒进行亚甲蓝光化学灭活处理,并用SYBR Green I荧光定量PCR对Sindbis病毒的cDNA进行扩增,同时以细胞病变法做平行对照以测定病毒残余滴度。结果显示在亚甲蓝光化学处理过程中,随着光照强度的增强,病毒残余滴度由6.50 LgTCID50/mL逐渐降低至检测限以下,同时病毒核酸的拷贝数显著下降(P<0.05),并与病毒感染性的降低呈线性相关(R2>0.98)。以上结果表明,亚甲蓝光化学灭活法对Sindbis病毒核酸有破坏作用,病毒核酸损伤程度随光照强度的增强而增加,且与病毒感染性的降低存在相关性,提示荧光定量PCR技术评价亚甲蓝光化学法的病毒灭活效果具有可行性。
|
| 关 键 词： | 荧光定量PCR 亚甲蓝光化学法 病毒灭活效果评价 |
Using Real-Time PCR to Evaluate the Effect of Viral Inactivation by Methylene Blue with Visible Light |
| |
| ZHANG Bo,ZHENG Lan,HUANG Yu-wen,MO Qin,WANG Xun,QIAN Kai-cheng.Using Real-Time PCR to Evaluate the Effect of Viral Inactivation by Methylene Blue with Visible Light[J].Chinese Journal of Virology,2009,25(4):286-290. |
| |
| Authors: | ZHANG Bo ZHENG Lan HUANG Yu-wen MO Qin WANG Xun QIAN Kai-cheng |
| |
| Institution: | 1.Shanghai Blood Center Graduate Student Training Base;East China Normal University;Shanghai 200051;China;2.Shanghai Blood Center;China |
| |
| Abstract: | To investigate the feasibility of using Real-Time PCR to evaluate the effectiveness of Sindbis virus inactivation by Methylene Blue with visible light.Sindbis virus was treated by Methylene Blue with different intensity of visible light and the transcribed cDNA was quantified by Real-Time PCR.Residual infectivity of treated virus was tested by cell infection method as parallel control at the same time.The residual infectivity of virus decreased from 6.50 lgTCID50/mL to under the limit of detection as light ... |
| |
| Keywords: | Real-Time PCR photochemical method of methylene blue with visible light evaluation of viral inactivation effect |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
