| 人类免疫缺陷病毒膜蛋白基因（env）F亚型毒株在中国的首例发现 |
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| 引用本文： | 潘品良,曾常红.人类免疫缺陷病毒膜蛋白基因（env）F亚型毒株在中国的首例发现[J].病毒学报,1999,15(2):97-101. |
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| 作者姓名： | 潘品良 曾常红 |
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| 作者单位： | [1]中国预防医学科学院艾滋病参比室 [2]广东省卫生防疫站 |
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| 摘 要： | １９９８年广东某戒毒所ＨＩＶ阳性感染者的血样，经ＰＣＲ扩增未培养细胞内ＨＩＶ前病毒基因并对Ｃ２－Ｖ３区进行测序和分析，发现两例感染者与巴西Ｆ亚型毒株ＢＺ１６３的基因离散率小于４％；Ｎｅｉｇｈｂｏｒ－ｊｏｉｎｉｎｇ系统树表明，它们与Ｆ亚型聚在一起；通过异源双链泳动技术分析法（ＨＭＡ）分析，这两例感染者与Ｆ亚型形成明显的异源二聚体。上述各项研究结果表明，Ｆ亚型ＨＩＶ－１已传入我国。
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| 关 键 词： | HMA HIV 艾滋病 PCR 膜蛋白基因 |
IDENTIFICATION AND CHARACTERIZATION OF HUMAN IMMUNODEFICIENCY VIRUS TYPE 1
SUBTYPE F env GENE IN CHINA |
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| Pan Pinliang Zeng Changhong Fan Xiujuan Yao Jun,Xing Hui Feng Yi,Shao Yiming.IDENTIFICATION AND CHARACTERIZATION OF HUMAN IMMUNODEFICIENCY VIRUS TYPE 1
SUBTYPE F env GENE IN CHINA[J].Chinese Journal of Virology,1999,15(2):97-101. |
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| Authors: | Pan Pinliang Zeng Changhong Fan Xiujuan Yao Jun Xing Hui Feng Yi Shao Yiming |
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| Abstract: | The human immunodeficiency
virus type 1 (HIV-1) env gene was amplified by PCR from PBMCs separated from sera of two
seropositive drug users (GD9817, GD9818) in Guangdong Province. The amplified genes were
sequenced for their C2-V3 regions and analyzed by HMA. The sequences were compared with
representative genes of various HIV-1 subtypes. Both sequencing and HMA analysis showed
that the GD9817 and GD9818 are subtype F HIV-1. Their genetic distances to the Brazil subtype
F strain BZ163 are only 4%. On the phylogenetic tree, they cluster with F strains of HIV-1. |
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| Keywords: | Human immunodeficiency virus Genetic subtype Nested-polymerase chain reaction Heteroduplex mobility analysis (HMA) Sequence analysis Phylogenetic tree analysis |
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