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表达强毒pp38决定簇的马立克病病毒疫苗毒CVI988点突变株的构建和特性
引用本文:崔治中 Lee,LF.表达强毒pp38决定簇的马立克病病毒疫苗毒CVI988点突变株的构建和特性[J].病毒学报,1999,15(2):147-153.
作者姓名:崔治中 Lee  LF
作者单位:[1]扬州大学动医系 [2]美国农业部禽病和肿瘤学研究所
摘    要:用鸡马立克病病毒(MDV)强毒GA株的38kD磷蛋白(pp38)基因克隆DNA转染I型弱毒疫苗CAI988/Rispens株MDV感染的鸡胚成纤维细胞,再用能识别I型强毒pp38的单克隆抗体H19做免疫荧光试验,筛选到能在pp38基因上表达强毒株特异性抗原决定簇的定向点突变弱毒株CVI/rpp38。用^35S-蛋氨酸标记的细胞裂解物做免疫沉淀反应表明,单抗H19不能识别天然CVI988株MDV中的

关 键 词:马立克病病毒  38kD磷蛋白  点突变  MDV

CONSTRUCTION AND CHARACTERIZATION OF POINT-MUTATED MAREK'S DISEASE VIRUS VACCINE STRAIN CVI988 EXPRESSING THE VIRULENT EPITOPE ON ITS 38kD PHOSPHORYLATED PROTEIN
Cui Zhizhong,Lee L. F..CONSTRUCTION AND CHARACTERIZATION OF POINT-MUTATED MAREK''''S DISEASE VIRUS VACCINE STRAIN CVI988 EXPRESSING THE VIRULENT EPITOPE ON ITS 38kD PHOSPHORYLATED PROTEIN[J].Chinese Journal of Virology,1999,15(2):147-153.
Authors:Cui Zhizhong  Lee L F
Institution:Cui Zhizhong 1 Lee L. F. 2
Abstract:Chicken embryo fibroblast (CEF) monolayer infected with serotype I Marek's disease virus (MDV) vaccine strain CVI988 were transfected with virulent MDV pp38 gene clone plasmid DNA in lipofect AMI NE solution. A point-mutated recombinant CVI/rpp38, which was expressing a virulent epitope on pp38, was screened and cloned by indirect fluorescent antibody test with monoclonal antibody H19. Immunoprecipitation indicated that H19 could not recognize pp38 complex from native CVI988 strain but did recognize the pp38 complex from the recombinant mutant CVI/rpp38 as from other serotype I virulent MDV strains. Chickens inoculated with the recombinant CVI/rpp38 gave significant lower MDV-specific antibody titers than chickens inoculated with the native CVI988 strain virus did. It demonstrated again that the pp38 from virulent MDV may have immunodepressive effects.
Keywords:Marek's disease virus  38kD phosphorylated protein      Point mutation
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