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| 应用异源双链泳动分析法快速确定(HIV-1)基因亚型 | |
| 引用本文： | 姚均,潘品良,邢辉,赵全壁,苏玲,邵一鸣,张峰,陈秀珠.应用异源双链泳动分析法快速确定(HIV-1)基因亚型[J].病毒学报,1999,15(4):296-304. |
| 作者姓名： | 姚均 潘品良 邢辉 赵全壁 苏玲 邵一鸣 张峰 陈秀珠 |
| 作者单位： | 1. 生部艾滋病预防与控制中心艾滋病参比实验室,北京,100050 2. 新疆自治区卫生防疫站,新疆乌鲁木齐830001 3. 同济医科大学微生物学教研室,湖北,武汉,430030 |
| 摘 要： | 应用异源双链泳动分析法（Ｈｅｔｅｒｏｕｄｕｐｌｅｘ ｍｏｂｉｌｉｔｙ ａｓｓａｙ，ＨＭＡ），对来自我国云南、四川、新疆、浙江、广东、福建、天津７省市７３份ＨＩＶ感染者样品，进行了ＨＩＶ－１膜蛋白（ｅｎｖ）基因片段亚型分析。通过ＨＭＡ能确定亚的有７０份，占样品总数的９５．８９％（７０／７２）。其中Ａ亚型２．８６％（２／７０），Ｆ亚型２．８６％（２／７０），泰国Ｂ亚型１８．５８％（１３／７０），欧美Ｂ |
| 关 键 词： | 亚型 序列测定 系统树 |
| 文章编号： | 1000-8721(1999)04-0296-09 |
RAPID GENETIC SUBTYPING OF HIV-1 USING A HETERODUPLEX MOBILITY ASSAY (HMA) |
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| YAO Jun ,PAN Pin-liang ,XING Hui ,ZHAO Quan-bi,ZHANG Feng ,SUN Ling CHEN Xiu-zhu ,SHAO Yi-ming.RAPID GENETIC SUBTYPING OF HIV-1 USING A HETERODUPLEX MOBILITY ASSAY (HMA)[J].Chinese Journal of Virology,1999,15(4):296-304. | |
| Authors: | YAO Jun PAN Pin-liang XING Hui ZHAO Quan-bi ZHANG Feng SUN Ling CHEN Xiu-zhu SHAO Yi-ming |
| Institution: | YAO Jun 1,PAN Pin-liang 1,XING Hui 1,ZHAO Quan-bi,ZHANG Feng 2,SUN Ling 1 CHEN Xiu-zhu 3,SHAO Yi-ming |
| Abstract: | We investigated the DNA fragment diversity of HIV-1 env gene by means of heteroduplex mobility assay (HMA) genotyping. Samples of 73 HIV-1 infected individuals from Yunnan, Sichuan, Xinjiang, Zhejiang, Guangdong, Fujian and Tianjing of our country were studied. The HMA had successfully genotyped 95.89% (70/73) of these samples as follows 2.86% (2/70) subtype A, 2.86% (2/70) subtype F, 18.58% (13/70) subtype Thailand B, 8.56% (6/70) subtype USA B, 38.57% (27/70) subtype C and 28.57% (20/70) subtype E. In order to test the reliability of these results and analyse the reason of failure to genotyping three samples by HMA, these strains were sequenced and phylogenetically analysed, and the results showed a perfect concordance with the HMA, the concordance rate was 95.89% (70/73). Reason of being unable to genotype three samples by HMA is that they were highly divergent subtype as compared to related reference strains. It suggests that, to improve reliability of HMA, the representative reference subtype plasmid selection must be continuously revised to cover viral diversification. |
| Keywords: | HIV-1 HMA |
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