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鸡贫血病毒衣壳蛋白基因克隆、测序和比较
引用本文:何成强,丁乃峥,李景鹏,李云龙.鸡贫血病毒衣壳蛋白基因克隆、测序和比较[J].病毒学报,2003,19(2):179-182.
作者姓名:何成强  丁乃峥  李景鹏  李云龙
作者单位:1. 山东师范大学,生物系动物抗性实验室,济南,250014
2. 东北农业大学,生命科学学院,哈尔滨,150030
基金项目:黑龙江省科技厅科技攻关项目;;
摘    要:VP1 gene of chicken anemia virus in liver of infected chicken from Harbin was amplified by polymerase chain reaction and cloned into pUC18The recombinant vector was identified by restriction digestionThree clones were sequenced with sequencing kit ABI PRISM by TaKaRa CoLtdThe open reading frame of the VP1 gene is made up of 1,350bpIt encodes 449 amino acid residuesIt was found that there is an abundant Arg region from position 3 to 46Its isoelectric point is up to 107 because of its abundant basic amino acidsComparing this Harbin isolate with 26p4,Cux-1,82-2,Del-rose,A2,Connb,L-028,TR20,CIA-1,CAV15,2A9 showed there were 168 nucleoside differences which lead to 26 amino acid changes of the VP1The amino acid changes may influence the antigenic character of different VP1sIt was also found that there is a hyper-mutation region from amino acids position 139-157,its mutation frequency is up to 42% among these CAV's VP1s.It is necessary to research the function of this region

关 键 词:鸡贫血病毒  衣壳蛋白基因  克隆  测序
文章编号:1000-8721(2003)02-0179-04

Cloning and Sequencing of the Capsid Gene of Chicken Anemia Virus(CAV) of the Harbin Isolate and Its Comparison with Other CAV Strains
Abstract:
Keywords:chicken  anemia  virus  cloning  sequencing
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