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我国乙型肝炎患者乙肝病毒前核心基因的分析
引用本文:卫清,张菁.我国乙型肝炎患者乙肝病毒前核心基因的分析[J].病毒学报,1994,10(1):63-67.
作者姓名:卫清  张菁
作者单位:上海医科大学医学分子病毒室,上海市大华医院
摘    要:

关 键 词:乙型肝炎  乙型肝炎病毒  前核心基因

ANALYSIS OF HEPATITIS B VIRUS PRE-CORE REGION IN CHINESE HEPATITIS B PATIENTS
WeiQing zhang Qing Wen Yu-mei.ANALYSIS OF HEPATITIS B VIRUS PRE-CORE REGION IN CHINESE HEPATITIS B PATIENTS[J].Chinese Journal of Virology,1994,10(1):63-67.
Authors:WeiQing zhang Qing Wen Yu-mei
Abstract:ariations in the sequences of the Pre-core region of hepatitis B virus(HBV)genomes iso-lated from sera of chinese chronic hepatitis B patients were studied.The entire Pre -core re-gions of HBV DNA were amplified by polymerase chain reaction (PCR) from 8 anti-HBepositive and 2 HBeAg positive chronic carriers. The amplified fragment were either cloned intoM13mp19 bacteriophage vector and at least three clones from each sample were sequenced orsequenced by PCR-direct sequencing method.By comparing the Pre-core sequences to that ofa previously cloned chinese prototype HBV DNA sequece (pADR-I)10 types of Pre-coremutations were found Six out of 10 types have a TAG stop codon mutation at codon 28 ofthe pre-core region.Two types show mutation from G to A at nucleotide 1901.One typehas a mutation which eliminates the translational initiation codon of the Pre-core gene andanother one type has a point mutation of nucleotide 1824 from T to C which does not leadto amino acid alteration.Our data demonstrate that the most prevalent type of mutation inthe HBV Pre-core region among chinese patients is the generation of a translational stopcodon.
Keywords:Hepatitis B virus e-antigen  Hepatitis B virus Pre-core gene  Nucleotide sequencing Shanghai Dahua Hospital To whom correspondence and reprint requests should be sent  
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