| 人源抗狂犬病毒中和性全抗体在昆虫细胞中的表达 |
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| 引用本文： | 张世珍,梁米芳,郑海发,李维,董关木,侯云德.人源抗狂犬病毒中和性全抗体在昆虫细胞中的表达[J].病毒学报,2001,17(3):193-199. |
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| 作者姓名： | 张世珍 梁米芳 郑海发 李维 董关木 侯云德 |
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| 作者单位： | 1. 中国预防医学科学院病毒学研究所
2. 中国药品生物制品检定所疫苗一室 |
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| 摘 要： | 将来源于噬菌体抗体库的人源狂犬病毒糖蛋白特异性单抗G10Fab的基因 ,克隆入杆状病毒人源IgG抗体表达载体 ,通过转染将重组质粒导入昆虫细胞 ,以全抗体的形式表达了一株人源抗狂犬病毒基因工程抗体G10。用亲和层析的方法纯化了表达产物 ,经与一株鼠源糖蛋白特异性单抗竞争证实 ,该单克隆抗体特异性识别狂犬病毒糖蛋白 ,亲和力约为 10 -9M。体外中和实验证明 ,该单抗对狂犬病毒aG株具有体外中和活性
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| 关 键 词： | 狂犬病毒 中和抗体 全抗体表达 昆虫细胞表达 体外中和活性 |
| 文章编号： | 1000-8721(2001)03-0193-07 |
| 修稿时间： | 2001年2月13日 |
Expression of Human Neutralizing Antibody in the Form of Intact Antibody in Insect Cell |
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| ZHANG Shi-zhen,LIANG Mi-fang,ZHENG Hai-fa,DONG Guan-mu,HOU Yun-de.Expression of Human Neutralizing Antibody in the Form of Intact Antibody in Insect Cell[J].Chinese Journal of Virology,2001,17(3):193-199. |
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| Authors: | ZHANG Shi-zhen LIANG Mi-fang ZHENG Hai-fa DONG Guan-mu HOU Yun-de |
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| Institution: | ZHANG Shi-zhen 1 LIANG Mi-fang 1* ZHENG Hai-fa 2 DONG Guan-mu 2 HOU Yun-de 1 |
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| Abstract: | Previously,we have reported that the Fab genes of a neutralizing human recombinant antibody G10 to rabies virus gycoprotein were isolated by using phage display technology The heavy and light chain antibody genes were subcloned into Baculovirus human IgG expression cassette vector and linked with IgG Fc portion The recombinant human intact antibody to rabies virus was generated by transfecting the genes into Sf9 insect cells and expressed in Sf9 cells with recombinant baculovirus The expressed recombinant IgG antibody was affinity purified and the specific binding property to the glycoprotein of rabies virus was demonstrated by antigen captured ELISA and competition ELISA The neutralization function of the G10 antibody was investigated and confirmed by animal test |
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| Keywords: | rabies virus neutralizing antibody intact antibody baculovirus-insect expression system neutralizing ability in vitro |
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