| 中国大麦黄花叶病毒分离物的分子变异 |
| |
| 引用本文： | 施农农,陈剑平,M. J. Adams.中国大麦黄花叶病毒分离物的分子变异[J].病毒学报,1999,15(4):339-347. |
| |
| 作者姓名： | 施农农 陈剑平 M. J. Adams |
| |
| 作者单位： | 1. 浙江省农业科学院,浙江,杭州,310021
2. 英国洛桑试验站 |
| |
| 基金项目： | 欧盟ISC项目，国家科技攻关项目，农业部重点科研项目，国家自然科学基金，浙江省青年科技人才专项基金，浙江省科技厅资助项目 |
| |
| 摘 要： | １３个供试的中国和英国大麦黄花叶病毒（ＢａＹＭＶ）分离物，经ＲＮＡ１和ＲＮＡ２全基因组不同区域ＤＮＡ片段背地里单构象多态分析（ＳＳＣＰ），外壳蛋白基因和ＲＮＡ２ ７０ｋＤ基因５端７０５碱基序列分析，以及此７－５碱基ＤＮＡ片段限制性内切酶图谱分析结果，它们的ＲＮＡ１和ＲＮＡ２彼此无一相同，其中ＲＮＡ２变异比ＲＮＡ１更大。由于变异十分复杂，且没有规律性，因而当前通用的分子生物学技术尚不能简单地ＢａＹＭ
|
| 关 键 词： | 大麦黄花叶病毒 分子变异 单链构象多态性 序列分析 限制性切酶图谱 |
| 文章编号： | 1000-8721(1999)04-0339-09 |
MOLECULAR VARIATIONS OF CHINESE ISOLATES OF BARLEY YELLOW MOSAIC BYMOVIRUS |
| |
| SHI Nong-nong,CHEN Jian-ping,M. J. Adams.MOLECULAR VARIATIONS OF CHINESE ISOLATES OF BARLEY YELLOW MOSAIC BYMOVIRUS[J].Chinese Journal of Virology,1999,15(4):339-347. |
| |
| Authors: | SHI Nong-nong CHEN Jian-ping M J Adams |
| |
| Abstract: | DNA fragments amplified from the bipartite genome of thirteen Chinese and UK isolates of barley yellow mosaic bymovirus(BaYMV) by RT-PCR were analysed by single-strand conformation polymorphism(SSCP). Variations in the coat protein gene and the 5'-terminal part(c. 705bp) of the 70kD regions of RNA2 of these BaYMV isolates were also revealed by sequencing and restriction mapping. The results indicated that none of all thirteen isolates was identical each other at the molecular level. Differences in RNA2 were greater than those in RNA1. The variations were very complicated so that none of modern molecular techniques could simply correlate the variations at nucleotide level with pathogenicity or strain differentiation. |
| |
| Keywords: | Barley yellow mosaic bymovirus Molecular variation Single-strand conformation polymorphism Sequencing Restriction mapping |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
