Isolation and characterization of 20 polymorphic microsatellite loci in the migratory freshwater fish Leporinus obtusidens (Characiformes: Anostomidae) using 454 shotgun pyrosequencing |
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| Authors: | G V Villanova M Vera J Díaz P Martinez N B Calcaterra S E Arranz |
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| Institution: | 1. Instituto de Biología Molecular y Celular de Rosario (IBR) – CONICET, Universidad Nacional de Rosario, Ocampo y Esmeralda sede CCT, 2000 Rosario, Argentina;2. Area Biología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, 2000 Rosario, Argentina;3. Genetics Department, Faculty of Veterinary, University of Santiago de Compostela, 27002 Lugo, Spain;4. Laboratori d'Ictiologia Genètica, Department of Biology, Faculty of Sciences, University of Girona, 17071 Girona, Spain |
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| Abstract: | Twenty polymorphic microsatellite markers were developed for the Neotropical fish Leporinus obtusidens using a next generation sequencing approach and tested in two other characifomes species, Schizodon platae and Prochilodus lineatus. Microsatellite loci alleles in L. obtusidens ranged between 2 and 20 alleles per locus (mean = 5·7), with expected heterozygosity values ranging from 0·097 to 0·956 (mean = 0·578) and observed heterozygosity values ranging from 0·000 to 0·800 (mean = 0·400) in a sample of 20 specimens from the lower Paraná River (Argentina). Most of these markers will be a valuable tool for captive breeding and stocking programmes, as well as for analyses of population connectivity and genetic structure in this broadly distributed Neotropical migratory fish. |
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| Keywords: | boga molecular marker Neotropical fish Paraná River Prochilodus lineatus Schizodon platae |
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