| 黄芩组织培养同源四倍体的诱导 |
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| 引用本文： | 陈柏君,高山林,卞云云.黄芩组织培养同源四倍体的诱导[J].植物资源与环境学报,2000,9(1):9-11. |
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| 作者姓名： | 陈柏君 高山林 卞云云 |
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| 作者单位： | 中国药科大学,南京,210038 |
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| 基金项目： | 本研究为国家中医药管理局重点科研项目的一部分(课题编号973302) |
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| 摘 要： | 应用组织培养技术对黄芩进行多倍体诱导，结果表明：组织培养条件下，在培养基中添加一定浓度和秋水仙素，或者把带有绿色芽点的黄芩愈伤组织经０．２％秋水仙素溶液浸泡一定时间后再进行培养，均可诱发黄芩多倍体的产生，但以后效果较好，诱导率可达４０．０％，通过试管苗根尖染色体显微观察，鉴定出５０多个黄芩同源四倍体，为今后优良品种的选育打下基础。
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| 关 键 词： | 黄芩 同源四倍体 组织培养 秋水仙素 中药材 |
| 文章编号： | 1004-0978(2000)01-0009-03 |
The inducing of autotetraploid of Scutellaria baicalensis Georgi by tissue culture |
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| CHEN Bai-jun,GAO Shan-lin,BIAN Yun-yun.The inducing of autotetraploid of Scutellaria baicalensis Georgi by tissue culture[J].Journal of Plant Resources and Environment,2000,9(1):9-11. |
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| Authors: | CHEN Bai-jun GAO Shan-lin BIAN Yun-yun |
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| Abstract: | A study of inducing polyploidy of \%Scutellaria baicalensis \%Georgi in the process of tissue culture was made. The results indicated that autotetraploid of \%S. baicalensis \%Georgi can be induced by adding colchicine in medium in tissue culture, or by immersing the callus of \%S. baicalensis \%Georgi in 0.2% colchicine solution for a period of time prior to culture. The later method was proved to be a good way in producing autotetraploid, with an inducing ratio of 40%. More than 50 lines of autotetraploid of \%S. baicalensis \%Georgi were determined by observing the chromosomes of obtained plantlets under microscope, and this laid down the foundation for the selecting and breeding of good varieties of \%S. baicalensis \%Georgi. |
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| Keywords: | Scutellaria baicalensis \%Georgi autotetraploid tissue culture colchicine |
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