| 山姜属中药草豆蔻和益智nrDNA ITS区序列的测定 |
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| 引用本文： | 赵志礼,周开亚,王峥涛,秦民坚,董辉,徐珞珊.山姜属中药草豆蔻和益智nrDNA ITS区序列的测定[J].植物资源与环境学报,2000,9(3):38-40. |
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| 作者姓名： | 赵志礼 周开亚 王峥涛 秦民坚 董辉 徐珞珊 |
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| 作者单位： | 1. 中国药科大学生药学研究室,南京,210038
2. 南京师范大学遗传资源研究所,南京,210097 |
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| 基金项目： | 国家自然科学基金资助项目(39600010)及江苏省资 |
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| 摘 要： | 中药草豆蔻、益智的原植物分别为山姜属草豆蔻（Ａｌｐｉｎｉａ ｈａｉｎａｎｅｎｓｉｓ Ｋ．Ｓｃｈｕｍ．）与益智（Ａ．ｏｘｙｐｈｙｌａ Ｍｉｑ．）。本文采用ＰＣＲ直接测序法，首次测定了它们的核糖体ＤＮＡ ＩＴＳ区序列。结果显示，两者序列长度（ＩＴＳ１＋ＩＴＳ２）分别为４０３ｂｐ与４０４ｂｐ，序列间具有２７个变异位点（包括５．８Ｓ编码区）。本研究为山姜属中药材的ＤＮＡ分子鉴定提供了必要的序列资料。
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| 关 键 词： | 山姜属 草豆蔻 益智 核糖体DNA ITS区序列 |
| 文章编号： | 1004-0978(2000)03-0038-03 |
| 修稿时间： | 1999年12月16 |
Determination of nrDNA ITS sequences of Caodoukou (Alpinia hainanensis K. Schum. ) and Yizhi(A. oxyphylla Miq.) |
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| ZHAO Zhi-li,ZHOU Kai-ya,WANG Zheng-tao,QIN Min-jian,DONG Hui,XU Luo-shan.Determination of nrDNA ITS sequences of Caodoukou (Alpinia hainanensis K. Schum. ) and Yizhi(A. oxyphylla Miq.)[J].Journal of Plant Resources and Environment,2000,9(3):38-40. |
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| Authors: | ZHAO Zhi-li ZHOU Kai-ya WANG Zheng-tao QIN Min-jian DONG Hui XU Luo-shan |
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| Abstract: | As the traditional Chinese medicines, Caodoukou (Semen Alpiniae Katsumadai) is from \%Alpinia hainanensis \%K. Schum., and Yizhi (Fructus Alpiniae Oxyphyllae) from \%A. oxyphylla \%Miq. Their nuclear ribosomal DNA internal transcribed spacer (ITS) regions were first sequenced. Sequence analysis showed that the ITS region (ITS 1 ITS 2) of \%A. hainanensis \%is 403 bp in length and that of \%A. oxyphylla \%is 404 bp. There are 27 variable sites (including 5.8 S coding region) in pairwise comparison of the sequences. This study could provide significant data for identifying the medicines at DNA level. |
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| Keywords: | Alpinia Roxb Alpinia hainanensis K Schum Alpinia oxyphylla Miq nrDNA ITS sequence |
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