美丽箬竹对模拟大气O3浓度倍增胁迫的生理响应

运用开顶式气室(OTCs)模拟当前环境大气O3浓度(对照,40～45 nL·L-1)、O3浓度倍增4倍(TR-1,92～106 nL·L-1)和O3浓度倍增2倍(TR-2,142～160 nL·L-1)胁迫,以叶片光合色素、MDA、可溶性蛋白质和可溶性糖含量及SOD和POD活性和相对电导率为指标,分析了美丽箬竹(Indocalamus decorus Q.H.Dai)对O3胁迫的生理响应规律.结果显示:随O3浓度的提高,叶片叶绿素a(Chla)、叶绿素b(Chlb)、总叶绿素和类胡萝卜素含量,SOD和POD活性,可溶性蛋白质和可溶性糖含量及二者的比值(SPC/SSC)均呈下降趋势；叶绿素a口与b的比值(Chla/Chlb)、MDA含量和相对电导率均呈增加趋势.TR-1条件下叶片POD活性和可溶性糖含量分别比对照下降38.86％和10.37％,差异显著；Chla/Chlb比值显著高于对照,其余指标均与对照无显著差异.而在TR-2条件下叶片Chla、Chlb、总叶绿素和类胡萝卜素含量显著低于对照,降幅分别为26.89％、40.91％、30.47％和20.37％；SOD和POD活性、可溶性蛋白质和可溶性糖含量以及PC/SSC比值也均显著低于对照,降幅分别为42.03％、46.62％、45.09％、11.52％和40.15％；Chla/Chlb比值、MDA含量和相对电导率均显著高于对照.另外,TR-1与TR-2处理组间光合色素含量和POD活性差异不显著,TR-2处理组MDA含量和相对电导率显著高于TR-1处理组,SOD活性显著低于后者.研究结果表明:美丽箬竹对O3胁迫具有强耐受性,可种植于O3浓度较高的环境中.

Physiological response of Indocalamus decorus to simulated atmospheric ozone stress with multiply-increasing concentrations

The method of open-top chambers(OTCs) was used to simulate ozone stress with atmospheric O3 concentration(control,40-45 nL·L-1),one time O3 concentration(TR-1,92-106 nL·L-1) and two times O3 concentration(TR-2,142-160 nL·L-1),and taking contents of photosynthetic pigment,MDA,soluble protein and soluble sugar,activities of SOD and POD and relative electric conductivity as indexes,physiological response of Indocalamus decorus Q.H.Dai to O3 stress was researched.The results show that with rising of O3 concentration,contents of chlorophylla(Chla),chlorophyllb(Chlb),total chlorophyll and carotenoid,activities of SOD and POD,soluble protein content(SPC) and soluble sugar content(SSC) and SPC/SSC value in leaves of I.decorus appear the reducing trend,while Chla/Chlb value,MDA content and relative electric conductivity appear the enhancing trend.In TR-1 group,POD activity and SSC decrease respectively 38.86% and 10.37% comparing to the control with the significant difference,Chla/Chlb value is significantly higher than that of the control,but the other indexes have no significant difference with the control.In TR-2 group,contents of Chla,Chlb,total chlorophyll and carotenoid are significantly lower than those of the control with decreasing ranges of 26.89%,40.91%,30.47% and 20.37%,respectively,activities of SOD and POD,SPC and SSC and SPC/SSC value also are significantly lower than those of the control with decreasing ranges of 42.03%,46.62%,45.09%,11.52% and 40.15%,respectively,but Chla/Chlb value,MDA content and relative electric conductivity are significantly higher than those of the control.Moreover,photosynthetic pigment content and POD activity have no significant differences between TR-1 and TR-2 groups,but MDA content and relative electric conductivity in TR-2 group are significantly higher and SOD activity in TR-2 group is significantly lower than those in TR-1 group.It is revealed that I.decorus possesses a strong resistance to O3 stress,and can be cultivated in high O3 concentration environment.