| 湿地松体细胞胚胎发生和植株再生 |
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| 引用本文： | 唐巍,郭仲琛.湿地松体细胞胚胎发生和植株再生[J].植物资源与环境学报,1997,6(2):8-11. |
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| 作者姓名： | 唐巍 郭仲琛 |
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| 作者单位： | [1]中国科学院化工冶金研究所生化工程国家重点实验室 [2]中国科学院植物研究所 |
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| 基金项目： | 国家“863”课题资助 |
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| 摘 要： | 以湿地松的未成熟合子胚为外植体，在附加８ｍｇ／Ｌ，２，４－Ｄ和４ｍｇ／Ｌ ＢＡ的ＬＰ培养基上诱导出胚性愈伤组织。在含１ｍｇ／Ｌ，２，４－Ｄ和０．５ｍｇ／Ｌ ＢＡ的培养基上保持并增殖。提高培养基的渗透压后，愈伤组织内大量的胚性胚柄细胞团和早期原胚。
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| 关 键 词： | 湿地松 体细胞胚胎发生 植株再生 |
Plantlet regeneration via somatic embryogenesis in slash pine |
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| Tang Wei and Ouyang Fan.Plantlet regeneration via somatic embryogenesis in slash pine[J].Journal of Plant Resources and Environment,1997,6(2):8-11. |
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| Authors: | Tang Wei and Ouyang Fan |
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| Abstract: | Embryogenic callus was initiated from immature zygotic embryos of slash pine ( Pinus elliottii Engelm.) cultured on LP medium containing 8 mg/L 2,4 D and 4 mg/L BA. Multiplication can be achieved on LP medium with 1 mg/L 2,4 D and 0.5 mg/L BA. Embryonal suspensor masses and early stage proembryos were formed from embryogenic callus cultured on high osmotic pressure medium. Early stage embryos developed into cotyledonary embryos on LP medium supplemented with 4 mg/L ABA, 75 g/L PEG and 5 g/L activated charcoal. Mature somatic embryos germinated into regeneration plantlet on medium with hormone free. The frequency of somatic embryos converted into regeneration plantlets is 15.6%. |
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| Keywords: | Pinus elliottii Engelm somatic embryogenesis plantlet regeneration |
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