| 大蒜分生组织培养脱病毒和快速繁殖技术 |
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| 引用本文： | 高山林,金雍安,蔡朝晖,刘立军.大蒜分生组织培养脱病毒和快速繁殖技术[J].植物资源与环境学报,2000,9(3):15-18. |
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| 作者姓名： | 高山林 金雍安 蔡朝晖 刘立军 |
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| 作者单位： | 1. 中国药科大学遗传育种教研室,南京,210038
2. 江苏外贸进出口集团有限公司,南京,210001 |
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| 基金项目： | 江苏外贸进出口集团有限公司委托和部分资助科研项目 |
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| 摘 要： | 采用茎尖分生组织培养技术，获得了大蒜（Ａｌｌｉｕｍ ｓａｔｉｖｕｍ Ｌ．）的无病毒试管苗。通过基本培养基和激素配比试验，筛选出最佳的培养基组成，进行脱病毒苗的快速繁殖。结果表明：诱导愈伤组织的最适培养基为：ＭＳ＋ＢＡ０．２ｍｇ／Ｌ＋ＮＡＡ０．５ｍｇ／Ｌ，月生长率达１２．７０倍；诱导丛生芽的最适培养基为：Ｂ５＋ＢＡ０．５ｍｇ／Ｌ＋ＩＡＡ０．２ｍｇ／Ｌ，丛生芽繁殖系数高达２５．５倍，技术上达到了快速繁
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| 关 键 词： | 大蒜 脱病毒 分生组织培养 快速繁殖 |
| 文章编号： | 1004-0978(2000)03-0015-04 |
| 修稿时间： | 2000年4月17日 |
Virus-free culture and rapid-propagation from shoot apex of Allium sativum L. |
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| GAO Shan-lin,JIN Yong-an,CAI Zhao-hui,LIU Li-jun.Virus-free culture and rapid-propagation from shoot apex of Allium sativum L.[J].Journal of Plant Resources and Environment,2000,9(3):15-18. |
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| Authors: | GAO Shan-lin JIN Yong-an CAI Zhao-hui LIU Li-jun |
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| Abstract: | Virus\|free materials of \%Allium sativum \%L. were obtained from \%in vitro \%shoot apex tissue culture. A series of optimization experiment for cultural medium composition, concentration of phytohormones were investigated with a view to accelerate the propagation of virus\|free materials. The obtained results indicated that the optimized medium for inducing callus was MS BA 0.2 mg/L NAA 0.5 mg/L. The growth rate of callus in one month was 12.70. The best medium for multiplying clumpy buds was B\-\{5\} BA 0.5 mg/L IAA 0.2 mg/L, the highest propagating ratio reached 25.5. These results could meet the technology requirements of rapid\|propagation in large scale production. Virus \|free materials were observed repeatedly under electron microscope, 5 virus\|free samples were tested out which could be used to fast propagation for production. |
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| Keywords: | Allium sativum \%L virus\|free shoot apex tissue culture |
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